Instrumentation for Computer-Based Detection and Imaging of Biological Samples

计算机辅助分析已经发展到这样一个地步：对现代生物学研究的进行来说，获得几种先进技术是或很快将是必不可少的。同位素探测灵敏度范围的进步以及数字化、记录和分析数据的能力已经达到这样的水平：计算机辅助数据的获取、分析和存储不再是一种奢侈，而是那些希望保持与时俱进和竞争力的人所必需的。基于计算机的电泳分析仪和荧光粉成像仪将作为共享设备，为生物系的成员提供数据采集，分析和存储的主要功能。同位素检测灵敏度的提高和各种样品介质的数字化，使人们有机会探索以前由于检测方法的限制而被认为无法回答的问题。这些技术的进步反过来又提供了大量的数据，这些数据往往会使人工分析甚至传统的软件程序不堪重负。这些拟议收购的目标是使尽可能多的参与科学家有效地使用单个实验室无法提供的仪器。要求资金用于购买(i)光学电泳分析仪，包括CCD相机、工作站和配套软件；（ii）荧光粉成像系统，包括扫描模块、工作站和配套软件。该部将提供其他必要设备，包括荧光粉储存屏、辅助软件和安装以太网网络系统，以便该部人员最大限度地利用所有设备。设备将由博士监督。罗伊·柯蒂斯三世和罗伯特·兰狄克。将安排研究生、博士后助理和教师使用，以便最大限度地利用设备促进所有参与者的研究进展。

Curtiss Computer assisted analysis has evolved to a point that access to several advanced technologies is, or soon will be, indispensable for the conduct of modern biological research. Advances in the sensitivity ranges of isotopic detection and the ability to digitize, record and analyze data has reached the level where computer assisted acquisition, analysis and storage of data is no longer a luxury but a necessity required by those wishing to remain current and competitive. The Computer-Based Electrophoresis Analyzer and Phosphor Imager will serve as shared equipment to provide the primary functions of data acquisition, analysis, and storage for members of the Department of Biology. Increased sensitivity of isotopic detection and digitization of a wide range of sample media have resulted in the chance to explore questions previously considered unanswerable due to limitations of detection methods. These technological advances in turn provide a formidable amount of data which can often overwhelm manual analysis and even traditional software programs. The goal of these proposed acquisitions is the efficient use by the maximum number of participating scientists of instruments which cannot be afforded in individual laboratories. Funds are requested to purchase (i) an Optical Electrophoresis Analyzer, consisting of CCD Camera, workstation, and accompanying software, and (ii) a phosphor imaging system consisting of scanning module, workstation, and accompanying software. The Department will provide other essential equipment, including phosphor storage screens, auxiliary software, and installation of the Ethernet networking system to allow maximum usage of all equipment by those in the Department. The equipment will be supervised by Drs. Roy Curtiss III and Robert Landick. Use by graduate students, post-doctoral associates, and faculty will be scheduled so as to make maximum use of the equipment to advance research progress by all participants.