RUI: Acquisition of Inverted and Upright Light Microscopes and an Image Processing and Analysis System to Support Research in Cell Biology

9317873 Koch光显微镜研究是细胞生物学研究的基石。任何一个细胞生物学研究实验室都不应该没有现代光学显微镜技术的完整补充，用于分析细胞结构和功能。这项建议寻求配套资金，以购买一台倒置显微镜和一台直立显微镜，每台显微镜都有相位对比、落射荧光和微分干涉对比光学器件，具有静止或视频显微摄影能力，所有这些都将连接到最先进的图像采集和分析系统以及专用图像处理计算机。该仪器将成为一个新的光学显微镜实验室的核心。 它的收购继续我们的努力，以提高教师，研究生和本科生学习细胞生物学的研究机会。所要求的那种显微镜目前还没有现成的。研究人员必须借用时间在一个单一的研究级直立显微镜或竞争与学生在五门课程的四个学生级直立显微镜-没有研究级倒置显微镜或图像采集/处理仪器是可用的。所要求的仪器将促进被囊动物精子和卵子激活的细胞生物学研究、海兔神经元中mRNA的轴突运输、植物对紫外线损伤的保护以及其他五个小项目。

9317873 Koch Light microscopic studies comprise the cornerstone of cell biological investigations. No cell biology research laboratory should be without access to the full complement of modern light microscopy techniques for analyzing cell structure and function. This proposal seeks matching funds to purchase one inverted microscope and one upright microscopes each having phase contrast, epifluorescence and differential interference contrast optics with still or video photomicrography capabilities all to be coupled to a state-of-the-art image acquisition and analysis system and a dedicated image processing computer. This instrumentation will form the nucleus of a new Light Microscopy Laboratory. Its acquisition continues our effort to enhance research opportunities for faculty, graduate and undergraduate students studying cell biology. Microscopes of the types requested are not readily available at present. Researchers must borrow time on a single research-grade upright microscope or compete with students in five courses for four student grade upright microscopes--no research-grade inverted microscope or image acquisition/processing instrumentation is available at all. The requested instrumentation will promote studies of cell biology of tunicate sperm and egg activation, axonal transport of mRNA in sea hare neurons, and protection to UV damage in plants as well as five other minor projects.