Genetic and Environmental Factors that Influence Microgametophytic Variation

影响小配子体变异的遗传和环境因素

• 批准号: 9318224
• 负责人: Andrew Stephenson
• 金额: $ 9.5万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318224&HistoricalAwards=false
• 关键词: Genetic; Environmental; Factors; Influence; Microgametophytic

9318224 Stephenson A large portion of the genome of the microgametophytes (pollen) is both transcribed and translated during development, germination and pollen tube growth. Consequently, the performance of pollen (speed and germination, pollen tube growth rate and the ability to penetrate ovules and achieve fertilization) can potentially be affected by its genetic composition, by its interactions with the pistil, by environmental conditions and by the resources provided to pollen by the pollen producing parent. This proposal seeks support to continue our detailed investigations of the factors that affect pollen performance, the relationship between pollen performance and non-random fertilization, and the effects of non-random fertilization on the progeny performance. The investigators will continue ongoing studies of pollen competition in Cucurbita texana, to determine if there is heritable variation for pollen performance and to determine if the effects of pollen competition on progeny vigor extend to subsequent generations. They will also determine the potential for pollen competition to occur and the effects of pollen competition on progeny vigor in natural populations of C. foetidissima growing in the Jornada Long-Term Ecological Research Site near Las Cruces, New Mexico. They will also determine if the growing conditions of the pollen producing parent affect the performance of the pollen. They will use C. texana and C. foetidissima to examine the effects of leaf herbivory by cucumber beetles (Diabrotica spp.) on pollen production, pollen grain size and the ability of the pollen to sire seeds when deposited into stigmas in mixture. This research will conclude studies of pollen competition and its effects on progeny performance and will result in the most carefully controlled and comprehensive set of experiments in this field of research. The herbivory studies will greatly extend the now very limited understanding of environmental factors that affect pollen performance.

小行星9318224斯蒂芬森 小配子体（花粉）的大部分基因组在发育、萌发和花粉管生长过程中进行转录和翻译。 因此，花粉的性能（速度和萌发、花粉管生长速率和穿透胚珠并实现受精的能力）可能受到其遗传组成、其与雌蕊的相互作用、环境条件和产生花粉的亲本提供给花粉的资源的影响。 该建议寻求支持，以继续我们的详细调查影响花粉性能的因素，花粉性能和非随机受精之间的关系，以及非随机受精对后代性能的影响。 研究人员将继续进行正在进行的德克萨斯南瓜花粉竞争研究，以确定花粉性能是否存在遗传变异，并确定花粉竞争对后代活力的影响是否延伸到后代。 他们还将确定花粉竞争发生的可能性以及花粉竞争对自然群体中的后代活力的影响。生长在新墨西哥州拉斯克鲁塞斯附近的Jornada长期生态研究基地。 他们还将确定产花粉亲本的生长条件是否影响花粉的性能。 他们将使用C。texana和C. foetidissima研究黄瓜叶甲（Diabrotica spp.）对花粉产量、花粉粒大小和花粉在混合物中沉积到柱头时产生种子的能力的影响。 这项研究将总结花粉竞争及其对后代表现的影响的研究，并将导致在这一研究领域的最仔细控制和全面的实验。 食草动物的研究将大大扩展现在非常有限的理解环境因素影响花粉的性能。