Bioremediation of Diaryl Ethers by Pseudomonas strain POB 310

9318788德怀尔这是一个为研究提供支持的奖项，其目的是确定在哪些条件下能够利用已被证明能够降解土壤有机污染物的细菌来对土壤进行田间范围的去污。这位研究人员计划研究的目标有机化合物是二芳基醚，这类化合物包括二恶英，这是一种具有重大污染意义的物质。研究人员计划使用的生物--假单胞菌产碱假单胞菌--已被发现能降解二芳基醚，这一特性可归因于其包含的酶基因，这些酶能裂解这些化合物的醚键。这项研究的结果预计将包括该生物体及其酶的底物范围，适合在野外规模操作中使用的条件，以及其降解目标物质的基因的稳定性。这项研究有望为评价微生物用于土壤去污的条件提供指导，并为从土壤中去除目标污染物的系统的工程设计提供依据。***

9318788 Dwyer This is an award to provide support for research, the objective of which is to determine conditions under which bacteria that have been shown to be capable of degrading organic contaminants of soil can be utilized in field-scale decontamination of soil. The target organic compounds this investigator plans to work on are diaryl ethers, compounds that as a class include the dioxins, substances of great pollutional significance. The organism the investigator plans to use, Pseudomonas pseudoalcaligenes, has been found to degrade diaryl ethers, a property attributable to its containing genes for enzymes that cleave these compound's ether bond. Results of this research are expected to include the substrate range for this organism and its enzymes, the conditions that are appropriate for its use in field-scale operations and the stability of its genes for degradation of the target substances. This research is expected to provide guidance for the conditions under which microorganisms may be evaluated for their use in decontamination of soils and provide the basis for engineering design of systems to remove the target contaminants from soil. ***