Transformation of Chickpea with Bt Genes, U.S.-Pakistan Cooperative Research

美国-巴基斯坦合作研究用 Bt 基因转化鹰嘴豆

• 批准号: 9320083
• 负责人: Milton Gordon
• 金额: $ 2万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9320083&HistoricalAwards=false
• 关键词: Transformation; Chickpea; Bt; Genes; U.S.

9320083 Gordon Description: This project is for a collaborative research by two well known laboratories: Dr. Milton Gordon's Laboratory, University of Washington, Seattle, and Dr. S. Riazuddin's Laboratory at the Centre for Advanced Molecular Biology, University of Punjab, Lahore, Pakistan. The work seeks to develop plant transformation procedures which will be applied to chickpea for the expression of Bacillus thuringiensis pesticidal proteins possessing entomocidal properties against American bollworm (Heliothis armigera). The study will address three main objectives. Firstly, pesticidal genes identified in Bt isolates from Pakistani environments will be cloned in plant expression vectors under the control of specific promoters. Secondly, procedures will be developed for the transformation of chickpea by a reporter gene (GUS) using particle bombardment. Finally, these procedures will be applied to transform chickpea with Bt pesticidal genes and the transgenic plant will be examined for level expression of the transgene and insect resistance. Scope: The project will provide fund for a young scientist from Dr. Riazuddin Laboratory to spend several months at the University of washington to participate with Dr. Gordon in the experiments using facilities not available in Lahore. This collaborative research is expected to be quite beneficial to the two laboratories, and particularly useful in the efforts towards producing isect-resistant economic crops. ***

小行星9320083 产品描述：该项目是由两个著名的实验室合作研究：西雅图华盛顿大学的米尔顿戈登博士实验室，和S。 巴基斯坦拉合尔旁遮普大学高级分子生物学中心Riazuddin实验室。 这项工作旨在开发植物转化程序，这将适用于鹰嘴豆的表达苏云金芽孢杆菌杀虫蛋白具有杀虫特性，对美国棉铃虫（棉铃虫）。 这项研究将涉及三个主要目标。 首先，将从巴基斯坦环境中分离的Bt菌株中鉴定的杀虫基因克隆到植物表达载体中，并置于特异性启动子的控制下。 其次，将开发程序的报告基因（GUS）的鹰嘴豆转化使用粒子轰击。 最后，将这些程序应用于用Bt杀虫基因转化鹰嘴豆，并检查转基因植物的转基因表达水平和抗虫性。 适用范围：该项目将为Riazuddin博士实验室的一名年轻科学家提供资金，让他在华盛顿大学呆上几个月，与Gordon博士一起使用拉合尔没有的设施进行实验。 这项合作研究预计将对两个实验室非常有益，特别是对生产抗虫害经济作物的努力非常有用。 ***