U.S.-Brazil Science Program: Genetic Manipulation of Garlic(Allium sativum L.) in Vitro and in Plants

这份美国-巴西奖将支持威斯康星大学的Philipp Simon博士和巴西农业研究公司（EMBRAPA）的Amaury Buso博士之间的合作。本项目将建立合作研究，以推进大蒜（Allium Sativum L.）的离体和植物遗传操作。本研究将完善和扩大大蒜真种生产，开发大蒜细胞培养和遗传转化方法。大蒜是世界上一种重要的蔬菜作物，已经被驯化了几千年，但有性繁殖却不得而知。性重组的产生是有限的。由于目前还没有开发出允许外源基因引入的基因转化系统，因此可以生产出无病毒的大蒜，但无法插入用于交叉保护的病毒基因。美国研究人员的工作使有性繁殖和评估大蒜的变异成为可能，而巴西研究人员则在大蒜细胞培养和植物生产方面进行了广泛的研究。双方将交流专业知识和互补技能，以通过有性繁殖产生新的大蒜品种，并开发将外源基因引入大蒜的系统。这项研究将通过美国威斯康星农业研究服务局和EMBRAPA之间的互访来完成。＊＊＊

9403486 Simon This U.S.-Brazil award will support collaboration between Dr. Philipp Simon of the University of Wisconsin, and Dr. Amaury Buso of Empresa Brazileira de Pesquisa Agropecuaria (EMBRAPA). This project will establish cooperative research to advance the genetic manipulation of garlic (Allium Sativum L.) in vitro and in plants. The research will perfect and expand true garlic seed production and develop methods of garlic cell culture and genetic transformation. Garlic, which is an important vegetable crop the world over, has been domesticated for thousands of years but sexual reproduction has been unknown. Generation of sexual recombinants is limited. Because a system of genetic transformation has not been developed which would allow foreign genes to be introduced, a virus-free garlic can be produced, but viral genes for cross-protection cannot be inserted. The U.S. researcher's work has enabled sexual reproduction and evaluation of variation in garlic, while the Brazilian researchers have worked extensively with garlic cell culture and plant production. The two sides will exchange their expertise and complementary skills to generate new garlic variation from sexual reproduction and develop a system for introducing foreign genes into garlic. The research will be accomplished through an exchange of visits between the U. Wisconsin Agricultural Research Service and EMBRAPA. ***