Soluble Methane Monooxygenase Expression and Co-Oxidation Activity Under Nitrogen-Limited Conditions (Collaborative Research)

9407286格雷厄姆这个项目的研究是通过增加甲烷营养细菌中可溶的甲烷单加氧酶的表达和活性来改善卤代化合物的生物修复。该策略是在不同的铜、氮和氧环境中培养野生型和突变菌株，同时监测sMMO和固氮酶的表达和活性。将对选定菌株的各种环境污染物的共氧化速率进行量化，并注意氮素限制的影响。这些污染物包括卤代乙烷、卤代甲烷和选定的杀虫剂。最后，表达和活动模式将通过一个模型与环境条件相关联，该模型将用于开发反应堆设计。***

9407286 Graham This project is on research to improve the bioremediation of halogenated compounds by increasing the expression and activity of the soluble methane monooxygenase (sMMO) enzyme in methanotrophic bacteria. The strategy is to culture wild-type and mutant strains of bacteria in various copper, nitrogen, and oxygen environments, while monitoring sMMO and nitrogenase expression and activity. The co-oxidation rates of a variety of environmental pollutants will be quantified for selected strains, with attention to the influence of nitrogen limitation. These pollutants include halogenated ethanes, halomethanes and selected pesticides. Finally, the expression and activity patterns will be related to the environmental conditions by a model which will be used to develop reactor designs. ***