Characterization of the Genes for Sulfate Activation from Higher Plants

高等植物硫酸盐激活基因的表征

• 批准号: 9408031
• 负责人: Thomas Leustek
• 金额: $ 14.96万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408031&HistoricalAwards=false
• 关键词: Characterization; Genes; Sulfate; Activation; Higher

9408031 Leustek Two enzymes, ATP sulfurylase and APS kinase, catalyze the activation of sulfate in plants leading to the synthesis of sulfur containing compounds vital for cell function. Isoenzymes of ATP sulfurylase are compartmentalized in chloroplasts, mitochondria and the cytosol, suggesting that they may play distinct physiological roles in sulfur metabolism. The objective of this research is to study the expression of the isoenzymes in order to establish a molecular basis for the control of sulfate activation. cDNAs encoding the sulfate activating enzymes were cloned from Arabidopsis thaliana by complementation of Saccharomyces cerevisiae ATP sulfurylase, met3, or APS kinase, met14, mutants. Four members of the ATP sulfurylase gene family and one encoding APS kinase have been isolated. It is proposed to further characterize these cDNAs and to study their expression and function in A. thaliana and Brassica juncea. Since sulfur-containing peptides play a significant role in resistance to heavy metals, the influence of heavy metal exposure will be investigated on expression of ATP sulfurylase and APS kinase. These studies will help advance our understanding of plant metabolism, growth, development and response to stress. ***

9408031 Leustek两种酶，ATP硫酸化酶和APS激酶，催化植物中硫酸盐的活化，导致合成对细胞功能至关重要的含硫化合物。 ATP硫酸化酶的同工酶在叶绿体、线粒体和胞质中都有分布，表明它们在硫代谢中可能起着不同的生理作用。 本研究的目的是研究同工酶的表达，以建立控制硫酸盐活化的分子基础。 通过酿酒酵母ATP硫酸化酶met3或APS激酶met14突变体的互补，从拟南芥中克隆编码硫酸活化酶的cDNA。 已分离出ATP硫酸化酶基因家族的四个成员和编码APS激酶的一个成员。 建议进一步表征这些cDNA并研究它们在A中的表达和功能。拟南芥和芥菜。 由于含硫肽在重金属抗性中起重要作用，因此将研究重金属暴露对ATP硫酸化酶和APS激酶表达的影响。 这些研究将有助于我们进一步了解植物的新陈代谢，生长，发育和对胁迫的反应。 ***