Modification, Functionalization, and Characterization of Carbon-Fiber Electrodes by Scanning Electrochemical Microscopy

该项目由分析和表面化学项目支持，重点研究了扫描电化学显微镜（SECM）和光学成像的应用，以表征碳纤维表面的非均匀性，以及SECM控制的对这些表面的修饰，以产生功能化的区域。这些微加工表面将被用于制造不寻常的基于氧化还原酶的传感器，这些传感器将被广泛评估。密西西比州立大学的David Wipf教授和他的学生，以及加州大学河滨分校的Werner Kuhr教授和他的学生，将在这项为期34个月的持续资助的任期内实现这些目标。电极表面的化学修饰可以使这些装置作为目标分析物的分子选择性传感器具有独特的应用。碳电极，特别是碳纤维微电极，为构建各种分子特异性电化学传感器提供了丰富的合成平台。本研究旨在了解用于这些应用的碳纤维表面的异质性，并开发氧化还原酶在这些表面上的空间分辨附着的协议。这项研究将导致具有广泛生物技术重要性的新型分析探针的发展。

This project, supported in the Analytical and Surface Chemistry Program, focusses on the application of scanning electrochemical microscopy (SECM) and optical imaging to characterizing the heterogeneity of carbon fiber surfaces and the SECM-controlled modification of these surfaces to produce regions which have been functionalized. These microfabricated surfaces will then be used to make unusual redox enzyme-based sensors which will be broadly evaluated. Professor David Wipf and his students at Mississippi State University, working in collaboration with Professor Werner Kuhr and his students at the University of California at Riverside, will pursue these goals during the tenure of this thirty-four month continuing grant. Chemical modification of the surface of electrodes can enable unique applications of these devices as molecularly selective sensors of target analytes. Carbon electrodes, particularly carbon fiber microelectrodes, offer a rich synthetic platform on which to build a wide variety of molecularly specific electrochemical sensors. This research is aimed at understanding the heterogeneity of surfaces of carbon fibers to be used in these applications and the development of protocols for the spatially resolved attachment of redox enzymes to these surfaces. This research should lead to the development of new analytical probes of broad biotechnological importance.