Anti-Idiotypic Antibodies as Probes of Plant Microtubule- Associated Proteins

抗独特型抗体作为植物微管相关蛋白的探针

• 批准号: 9415479
• 负责人: Louis Morejohn
• 金额: $ 5.5万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9415479&HistoricalAwards=false
• 关键词: Anti; Idiotypic; Antibodies; Probes; Plant

9415479 Morejohn This is a Small Grant for Exploratory Research. The project is a fishing expedition for microtubule-associated proteins (MAPs) in plants, using a high-risk, innovative approach based on the production of anti-idiotypic antibodies as reagents for identifying putative MAPs. %%% In animal systems, a lot is known about microtubule- associated proteins (MAPs), in terms of both protein structure and functions. These MAPs play critical roles in stabilizing microtubules and regulating the assembly and disassembly of microtubules. In plants, a generally accepted identification of any MAP has not yet been achieved. Since microtubules play critical roles in the fundamental life processes of plant cells, and since they are the targets of action of an important class of herbicides, there is compelling reason to increase our understanding of microtubule structure and function in plants, including the identification of MAPs. This project is a high-risk but plausible approach to the identification of plant MAPs. ***

这是一笔用于探索性研究的小额拨款。该项目是对植物中微管相关蛋白（MAPs）的一次“钓鱼考察”，采用一种高风险的创新方法，即生产抗独特型抗体作为鉴定推定MAPs的试剂。在动物系统中，人们对微管相关蛋白（MAPs）的结构和功能了解甚多。这些map在稳定微管和调节微管的组装和拆卸中起着关键作用。在植物中，对任何MAP的普遍认可的鉴定尚未实现。由于微管在植物细胞的基本生命过程中起着至关重要的作用，并且它们是一类重要除草剂的作用靶点，因此有充分的理由增加我们对植物微管结构和功能的了解，包括对MAPs的鉴定。该项目是一种高风险但可行的植物MAPs鉴定方法。＊＊＊