Assembly and Regulation of Myosin II in Endothelial Locomotion

;​你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，你是说，%,'() * +, -。/ F Microsoft Word 6.0 Document MSWordDoc6;​这个项目的目标是了解肌球蛋白II是如何参与运动和塑造内皮细胞的，内皮细胞是形成血管内壁的细胞。肌球蛋白II是一种蛋白质，它可以通过移动细胞内的其他分子来充当马达。为了了解这种产物如何在整个细胞内运动，肌凝蛋白II在细胞内的位置和运动将被定义。为此，将荧光染料附着在肌球蛋白II上，并将标记的肌球蛋白注射到培养皿中生长的细胞中。然后通过荧光显微镜观察标记的肌球蛋白II的组织和动力学。为了了解肌凝蛋白II的行为是如何被控制的，将检查运动细胞中的肌凝蛋白II在其位置或运动变化时发生的生化变化。这将通过制造只与改良形式的肌球蛋白II结合的抗体来完成。这些抗体可以用来给细胞染色，以显示生化变化发生的时间和地点。内皮细胞，像身体中的许多细胞一样，在正常发育、伤口愈合和许多疾病，特别是癌症中都是活动的。了解运动是如何在细胞和分子水平上发生和控制的，对于开发治疗伤口和疾病的新策略非常重要。*** ;Oh +' 0 $ H l D H R:\WWUSER\TEMPLATE\NORMAL。如果你是我的朋友，你会发现我是你的朋友，我是你的朋友9417115雪莉·帕克雪莉·帕克@ u @ @ u @ f#微软Word 6.0;e 3 e P 4 P j j j j j j j j x 1：zt7 x j x j j j j ~ j j j j j j r 9417115 Kolega这个项目的目标是了解肌球蛋白II是如何参与运动和塑造内皮细胞的，内皮细胞是形成血管内层的细胞。肌球蛋白II是一种蛋白质，它可以通过移动细胞内的其他分子来充当马达。为了了解这种产物如何在整个细胞内运动，肌凝蛋白II在细胞内的位置和运动将被定义。为此，将荧光染料附着在肌球蛋白II上，并将标记的肌球蛋白注射到培养皿中生长的细胞中。然后通过荧光显微镜观察标记的肌球蛋白II的组织和动力学。为了了解肌凝蛋白II的行为是如何被控制的，将检查运动细胞中的肌凝蛋白II在其位置或运动变化时发生的生化变化。这将通过制造只与改良形式的肌球蛋白II结合的抗体来完成。这些抗体可以用来给细胞染色，以显示生化变化发生的时间和地点。内皮细胞，像体内的许多细胞一样，在正常发育、伤口愈合和许多疾病，特别是癌症中都是活动的。了解运动是如何在细胞和分子水平上发生和控制的，对于开发治疗伤口和疾病的新策略非常重要。* * *

; R o o t E n t r y F { C o m p O b j b W o r d D o c u m e n t 4 O b j e c t P o o l { { % & ' ( ) * + , - . / F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; 9417115 Kolega The goal of this project is to understand how myosin II is involved in moving and shaping endothelial cells, the cells that form the inner lining of blood vessels. Myosin II is a protein that can act as a motor by moving other molecules within the cell. In order to understand how this products movement of the whole cell, the location and movements of myosin II inside of cells will be defined. To do this a fluorescent dye will be attached to myosin II and this labeled myosin injected into cells growing in culture dishes. The organization and dynamics of the labeled myosin II will then be followed through a fluorescence microscope. In order to understand how the behavior of myosin II is controlled, the myosin II in moving cells will be examined for biochemical changes that occur when its location or movement changes. This will be accomplished by making antibodies that bind only to modified forms of myosin II. These antibodies can be used to stain cells to show when and where biochemical changes occur. %%% Endothelial cells, like many cells in the body, move about during normal d evelopment, wound healing and in many diseases, particularly cancer. Understanding how movement occurs and is controlled at the cellular and molecular level is important for developing new strategies for treating wounds and disease. *** ; Oh +' 0 $ H l D h R:\WWUSER\TEMPLATE\NORMAL.DOT S u m m a r y I n f o r m a t i o n ( $ 9417115 Shirley Parker Shirley Parker @ u @ @ u @ F # Microsoft Word 6.0 2 ; e 3 e P 4 P j j j j j j j x 1 : Z T 7 x j x j j j j ~ j j j j r 9417115 Kolega The goal of this project is to understand how myosin II is involved in moving and shaping endothelial cells, the cells that form the inner lining of blood vessels. Myosin II is a protein that can act as a motor by moving other molecules within the cell. In order to understand how this products movement of the whole cell, the location and movements of myosin II inside of cells will be defined. To do this a fluorescent dye will be attached to myosin II and this labeled myosin injected into cells growing in culture dishes. The organization and dynamics of the labeled myosin II will then be followed through a fluorescence microscope. In order to understand how the behavior of myosin II is controlled, the myosin II in moving cells will be examined for biochemical changes that occur when its location or movement changes. This will be accomplished by making antibodies that bind only to modified forms of myosin II. These antibodies can be used to stain cells to show when and where biochemical changes occur. %%% Endothelial cells, like many cells in the body, move about during normal development, wound healing and in many diseases, particularly cancer. Understanding how movement occurs and is controlled at the cellular and molecular level is important for developing new strategies for treating wounds and disease. ***