Synthetic Ion Channels Based on Peptide-Supported Poly(Crown Ethers)

基于肽支撑的聚冠醚的合成离子通道

• 批准号: 9418362
• 负责人: James Hutchison
• 金额: $ 3.2万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-10-01 至 1995-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9418362&HistoricalAwards=false
• 关键词: Synthetic; Ion; Channels; Based; Peptide

9418362 Hutchison This starter grant award to the University of Oregon will support the research of Professor James E. Hutchison. The theme of the research will be the synthesis of functional ion channel models based on polypeptide-supported poly(crown ethers). The polypeptide serves to align stacks of crown ethers that form the pore. The unique property of this ion-channel model is the ease with which the model's structure can be systematically varied to probe the dependence of ion transport through the channel with the structure of the channel. The channel's inside diameter, the molecular composition of the channel's lining, the length of the channel, and the separation between crown ethers within the polymer will be varied to give structurally well-defined ion channel models. The nature of the ion transport through these ion channels will be tested by imbedding them in planar lipid bilayers and measuring the ion fluxes through them as a function of ions in solution and transmembrane potential. Ion channels selectively mediate ionic transport through cell membranes and play a major role in shaping biological electrical impulses. This research will advance the fundamental understanding of the structure/function relationship.

9418362 Hutchison俄勒冈州大学的启动资金将用于支持James E.哈奇森本论文的研究主题是基于多肽负载的聚冠醚的功能性离子通道模型的合成。多肽用于排列形成孔的冠醚的堆叠。该离子通道模型的独特性质是该模型的结构可以系统地改变以探测通过通道的离子传输与通道结构的依赖性的容易性。通道的内径、通道内衬的分子组成、通道的长度和聚合物内冠醚之间的间隔将变化，以给出结构明确的离子通道模型。通过这些离子通道的离子转运的性质将通过将它们嵌入平面脂质双层中并测量通过它们的离子通量作为溶液中的离子和跨膜电位的函数来测试。 离子通道选择性地介导离子通过细胞膜的运输，并在形成生物电脉冲中起主要作用。这项研究将促进对结构/功能关系的基本理解。