Multi-User Microscope for Analysis of Double-Label Epifluorescence

项目编号：IBN-9418641首席研究员：Cheryl Sisk该奖项旨在为密歇根州立大学的神经内分泌学家提供高分辨率的荧光显微镜。高结果荧光显微镜将用于定位生物分子到神经系统特定区域内单个细胞的特定亚室。这些分子被荧光标记，在紫外线下显示出特定的颜色。通过这种方式，不同的分子可以被标记为不同的颜色，以确定，例如，特定的一对分子是一起出现还是单独出现在特定的细胞中。这些科学家将使用显微镜同时检测活性依赖性标记物和类固醇受体蛋白，比较肥大细胞和神经胶质细胞中的酶表达模式，以及神经肽和神经递质在大脑不同区域的共定位。此外，这项技术可以用来确定当动物参与不同的行为时，这些分子的分布是如何变化的，例如，受到不同的光周期（如随季节发生的光周期）的影响。这台显微镜还将配备一个教学头，以便科学家们可以使用显微镜向本科生教授这些最先进的组织学和免疫细胞化学技术。

Proposal Number: IBN-9418641 Principal Investigator: Cheryl Sisk This award is to provide a high-resolution epifluorescence microscope for research neuroendocrinologists at Michigan State University. High-resultion fluorescence microscopy will be used to localize biological molecules to specific subcompartments of individual cells within particular areas of the nervous system. These molecules are tagged with fluorescent markers that show particular colors under ultraviolet light. That way different molecules can be tagged with different colors to determine, for example, if a particular pair of molecules occur together or separately inside specific cells. These scientists will use the microscope for concurrent detection of activity-dependent markers and steroid receptor proteins, for comparisons of enzyme expression patterns in mast cells and glia, and for colocalization of neuropeptides and neurotransmitters in different regions of the brain. Furthermore, this technique can be used to determine how the distribution of these molecules changes when the animal is involved in different behaviors or, for example, is subjected to different photoperiods such as those that occur with the seasons. This microscope will also be equipped with a teaching head so that the scientists can use the microscope for teaching undergraduates these state-of-the-art histological and immunocytochemical techniques.