Multi-User Microscope for Analysis of Double-Label Epifluorescence

提案编号：IBN-9418641主要研究者：Cheryl Sisk 该奖项旨在为密歇根州立大学的神经内分泌学家提供高分辨率的落射荧光显微镜。 高结果荧光显微镜将被用来定位生物分子的特定亚区的个别细胞内的特定区域的神经系统。 这些分子被荧光标记物标记，在紫外光下显示特定的颜色。 通过这种方式，不同的分子可以用不同的颜色标记，以确定特定的分子对是否在特定的细胞内一起或单独出现。 这些科学家将使用显微镜同时检测活性依赖性标记物和类固醇受体蛋白，比较肥大细胞和神经胶质细胞中的酶表达模式，并在大脑不同区域共定位神经肽和神经递质。 此外，该技术可用于确定当动物参与不同的行为或例如经受不同的光周期（如随季节发生的光周期）时这些分子的分布如何变化。 该显微镜还将配备教学头，以便科学家可以使用显微镜向本科生教授这些最先进的组织学和免疫细胞化学技术。

Proposal Number: IBN-9418641 Principal Investigator: Cheryl Sisk This award is to provide a high-resolution epifluorescence microscope for research neuroendocrinologists at Michigan State University. High-resultion fluorescence microscopy will be used to localize biological molecules to specific subcompartments of individual cells within particular areas of the nervous system. These molecules are tagged with fluorescent markers that show particular colors under ultraviolet light. That way different molecules can be tagged with different colors to determine, for example, if a particular pair of molecules occur together or separately inside specific cells. These scientists will use the microscope for concurrent detection of activity-dependent markers and steroid receptor proteins, for comparisons of enzyme expression patterns in mast cells and glia, and for colocalization of neuropeptides and neurotransmitters in different regions of the brain. Furthermore, this technique can be used to determine how the distribution of these molecules changes when the animal is involved in different behaviors or, for example, is subjected to different photoperiods such as those that occur with the seasons. This microscope will also be equipped with a teaching head so that the scientists can use the microscope for teaching undergraduates these state-of-the-art histological and immunocytochemical techniques.