UV-Visible Spectrophotometry for Chemistry and Biology

用于化学和生物学的紫外-可见分光光度法

• 批准号: 9450882
• 负责人: Steven Walstrum
• 金额: $ 0.61万
• 依托单位: University of Dubuque
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9450882&HistoricalAwards=false
• 关键词: UV; Visible; Spectrophotometry; Chemistry; Biology

9450882 Walstrum Environmental Science is a multidisciplinary program that attracts by far the largest number of science students at the University of Dubuque. The number of students in the program has increased dramatically in the last two years. One goal of the program is to train students in environmental analysis. Ultraviolet-visible spectrophotometry is widely used in analyzing inorganic substances (e.g., phosphate, chromium) in environmental samples. We will purchase a diode-array UV-visible spectrophotometer that can obtain a spectrum in seconds, and automatically acquire kinetic data. We propose to use the instrument in a number of classes in the chemistry and biology curriculum. In instructional labs, we will use the instrument largely for quantitative analysis of chemicals of environmental interest and examining the spectra of biological pigments, and we will also use it for kinetics. In students' independent projects, we will use the instrument for evaluating the effectiveness of certain enzymes in degrading pollutants by examining the kinetics of reaction. We believe that using environmental analysis in laboratory classes is an effective way of increasing student interest in science, and that the laboratory exercises that we are using can be adopted by any science program whether or not they have course offerings in environmental science or a major in environmental science.

Walstrum环境科学是一个多学科项目，迄今为止吸引了迪比克大学最多的理工科学生。在过去的两年里，这个项目的学生人数急剧增加。该项目的目标之一是培养学生的环境分析能力。紫外可见分光光度法广泛应用于分析环境样品中的无机物（如磷酸盐、铬）。我们将购买一个二极管阵列紫外可见分光光度计，可以在几秒钟内获得光谱，并自动获取动力学数据。我们建议在化学和生物课程的一些课程中使用该仪器。在教学实验室中，我们将主要使用该仪器对环境相关化学物质进行定量分析，并检查生物色素的光谱，我们也将使用它进行动力学研究。在学生的独立项目中，我们将使用该仪器通过检查反应动力学来评估某些酶降解污染物的有效性。我们相信，在实验课中使用环境分析是提高学生对科学兴趣的有效方法，我们使用的实验练习可以被任何科学项目所采用，无论他们是否有环境科学课程或环境科学专业。