CAREER: Nuclelar Magnetic Resonance Analysis of Protein Conformation During Bioprocessing

职业：生物加工过程中蛋白质构象的核磁共振分析

• 批准号: 9501909
• 负责人: Erik Fernandez
• 金额: $ 38万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9501909&HistoricalAwards=false
• 关键词: CAREER; Nuclelar; Magnetic; Resonance; Analysis

9501909 Fernandez This project is on research to exploit the detailed residue-level structural information available from nuclear magnetic resonance (NMR) to examine proteins in non-native environments relevant to manufacturing. Proteins will be examined in a variety of environments with the goal of developing a general understanding of protein denaturation during precipitation. Changes in conformation that occur in the solution state before aggregation/precipitation, conformation changes that occur upon phase change, and the rates of these changes will be examined with NMR. In addition to this research activity, the investigator will enhance education by engaging in activities on curricular development, laboratory development and mentoring. ****

9501909费尔南德斯这个项目正在进行研究，利用核磁共振提供的详细残基水平结构信息来检查与制造相关的非本地环境中的蛋白质。将在不同的环境中对蛋白质进行检测，目的是对蛋白质在沉淀过程中的变性有一个普遍的了解。将用核磁共振检查聚集/沉淀前溶液状态中发生的构象变化、相变时发生的构象变化以及这些变化的速率。除了这项研究活动外，调查员还将通过参与课程开发、实验室开发和指导活动来加强教育。****