Mechanisms of Electrode Reactions

该项目在分析和表面化学计划的支持下，旨在开发电化学方法来研究化学和生物化学问题。在为期三年的持续资助期间，德克萨斯大学奥斯汀分校的艾伦·J·巴德教授和他的学生将深入了解蛋白质结构、结合常数、化学计量，并通过利用电活性剂与生物分子的相互作用开发生物传感器。生物大分子与这些试剂相互作用引起的电流和电化学发光反应的变化将用于探测涉及酶，抗体和DNA的反应。此外，扫描电化学显微镜（SECM）将用于检测快速异质和耦合均相反应，并研究电子和离子在液-液界面上的传输，从而允许表征聚合物膜和自组装单层。 电化学方法已经被用于测量许多金属、有机、生物和半导体物质的组成、浓度和反应性。 这些技术通常用于工业和临床行业，以进行简单的测定，如游泳池中的水的pH值，饮用水中的污染物，以及识别体内复杂的痕量激素，这些激素是怀孕的信号。 德克萨斯大学奥斯汀分校的艾伦巴德教授和他的学生一直是电分析化学领域的领导者，他们将带头开发在生物技术、环境分析和先进制造等领域有用的新的分析和表面分析方法。

This project, supported in the Analytical and Surface Chemistry Program, is directed towards the development of electrochemical methods to study chemical and biochemical problems. During the tenure of this three-year continuing grant, Professor Allen J. Bard and his students at the University of Texas at Austin will gain insights into protein structure, binding constants, stoichiometry, and develop biosensors by exploiting the interaction of electroactive agents with biomolecules. Changes in current and electrochemiluminescence responses caused by interactions of biomacromolecules with these agents will be used to probe reactions involving enzymes, antibodies, and DNA. In addition, scanning electrochemical microscopy (SECM) will be used to detect fast heterogeneous and coupled homogeneous reactions and to investigate electron and ion transport across liquid-liquid interfaces, thus allowing characterization of polymer films and self-assembled monolayers. Electrochemical approaches have been used for generations to measure the composition, concentration, and reactivity of numerous metal, organic, biological and semiconductor species. These techniques are used routinely in industrial and clinical industries to make simple determinations such as the pH of water in swimming pools, contaminants in drinking water, and the identification of complex, trace level hormones in the body which signal pregnancy. Professor Allen Bard and his students at the University of Texas at Austin have been leaders in the field of electroanalytical chemistry and will be spearheading new analytical and surface analysis methods useful in such fields as biotechnology, environmental analysis, and advance manufacturing.