An Automated Nutrient Analyzer System for Biological Research

用于生物研究的自动营养分析仪系统

• 批准号: 9512945
• 负责人: Robert Petty
• 金额: $ 5.04万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512945&HistoricalAwards=false
• 关键词: Automated; Nutrient; Analyzer; System; Biological

This is a request for funds for the purchase of an automated nutrient analysis system. This would be a multi-user instrument, and would be housed in a shared-use analytical and instrumentation facility at UCSB's Marine Science Institute (MSI). The new analyzer would replace a ten-year-old system that no longer has the capabilities required to support current and proposed research projects. Knowledge of the concentrations of dissolved inorganic nutrients in aquatic systems is of fundamental importance to a wide range of biological studies. Areas of interest to researchers at MSI encompass both fresh and salt water systems, and have included such diverse areas as hydrological and ecological studies of Amazonian lake and river systems, nutrient dynamics in areas of merging water masses and coastal upwelling, biogeochemical processes in California's Sierra Nevada watershed, water quality and nutrient cycling studies in coastal estuaries, and ecological studies in both northern and southern polar regions. A11 of these studies have required the analysis of large numbers of water samples for up to five nutrient species. Nutrient analyses have been done using an analyzer system that is now over 10 years old, and which suffers from various problems, including unstable detectors, relatively poor detection limits, and general obsolescence. Some of the aforementioned projects are ongoing, while new projects requiring nutrient analyses are starting up and/or being proposed almost continuously. A feature they all have in common is the need for better and faster nutrient analysis. In the nutrient cycling studies of coastal marshes and estuaries, for example, a system is required that can automaticallv handle the wide range of concentrations (1 to 1000 uM) and salinities (O to 34 ppt) that are routinely encountered (error-prone manual dilutions and salini ty adjustments having been necessary in the past). In the programs dealing with polar systems, sample size (for logistical reasons) and analysis time (because of sample stability) are critical. And in a recently initiated long-term program to monitor the chemical, physical, and biological parameters influencing sedimentation in a coastal area featuring a high-resolution sedimentary record, the rapidly changing oceanographic conditions require timely nutrient analyses (to provide quick feedback to the project), with both high sensitivity (to detect depleted waters), and high precision (to follow subtle long-term changes). The requested analyzer system would fill all of these requirements. The system we are requesting is the Perstorp Flow Solution III, a continuous-flow analyzer with capabilities for both flow injection and segmented flow technologies. It would be configured for the simultaneous analysis of up to five nutrient species (phosphate, nitrate, nitrite, silica, and ammonia), and would include a random-access autosarnpler, peristaltic pump, injection valves, analysis cartridges for the , five nutrients, double-beam photometric detectors, and a PC-type computer/controller with Windows-based software.

这是购买自动化营养分析系统的资金请求。这将是一个多用户仪器，并将安装在 UCSB 海洋科学研究所 (MSI) 的共享分析和仪器设施中。 新的分析仪将取代使用了十年的系统，该系统不再具备支持当前和拟议的研究项目所需的功能。 了解水生系统中溶解的无机营养物的浓度对于广泛的生物学研究至关重要。 MSI 研究人员感兴趣的领域包括淡水和咸水系统，包括亚马逊湖泊和河流系统的水文和生态研究、合并水团和沿海上升流区域的营养动态、加利福尼亚内华达山脉流域的生物地球化学过程、沿海河口的水质和营养循环研究以及北极和南极地区的生态研究等不同领域。 这些研究中的 A11 需要对大量水样进行多达五种营养物质的分析。 营养成分分析使用的分析系统已有 10 多年的历史，该系统存在各种问题，包括检测器不稳定、检测限相对较低以及普遍陈旧。 上述一些项目正在进行中，而需要营养分析的新项目正在启动和/或几乎不断地提出。它们的一个共同特点是需要更好、更快的营养分析。例如，在沿海沼泽和河口的养分循环研究中，需要一个能够自动处理经常遇到的各种浓度（1 至 1000 uM）和盐度（0 至 34 ppt）的系统（过去需要进行容易出错的手动稀释和盐度调整）。在处理极性系统的程序中，样本量（出于后勤原因）和分析时间（因为样本稳定性）至关重要。在最近启动的长期计划中，监测影响具有高分辨率沉积记录的沿海地区沉积的化学、物理和生物参数，快速变化的海洋条件需要及时的营养物分析（为项目提供快速反馈），同时具有高灵敏度（检测枯竭水域）和高精度（跟踪微妙的长期变化）。 所要求的分析系统将满足所有这些要求。 我们需要的系统是 Perstorp Flow Solution III，这是一种连续流分析仪，具有流动注射和分段流动技术的功能。 它将配置为同时分析多达五种营养物质（磷酸盐、硝酸盐、亚硝酸盐、二氧化硅和氨），并包括随机访问自动采样器、蠕动泵、注射阀、五种营养物质的分析盒、双光束光度检测器和带有基于 Windows 软件的 PC 型计算机/控制器。