A CHNS Analyzer to Investigate Resource Use and Availability In Biotic Systems

该提案涉及购买分子动力学425SI磷光成像仪的申请，这是一种用于检测和定量各种生化实验中使用的放射性的设备。该仪器的定量和高分辨率能力将允许进行目前不可能进行的生化实验。与传统的使用X射线胶片的放射自显影技术相比，该技术的主要优点是增加了线性响应、动态范围和灵敏度，并缩短了曝光时间。该仪器提供了使用放射性同位素如32P、35S、14C和1251的实验的定量数据。目前，我们加州大学伯克利分校的大楼(Koshland Hall)还没有这种类型的乐器。放射性样品的定量将由用户组在第8节详细说明的各种生化和分子生物学实验中进行。定量分析的主要领域将是核酸-蛋白质(DNA和RNA)相互作用和信号转导(信号蛋白质的GTPase活性)。这项技术涉及使用储存式荧光屏，它存储了样品贝塔粒子发射的能量。然后，通过用激光扫描存储屏幕来释放存储的能量。这种发射由仪器检测并存储在计算机硬盘上。然后，可以使用随仪器提供并通过以太网络传输到用户计算机终端的软件包来访问和分析数据。一些目前不可能进行的定量生化实验将是可行的，因为分子动力学磷光成像仪远远优于X射线胶片放射自显影。

This proposal involves a request to purchase the Molecular Dynamics 425SI Phosphorimager, a device for the detection and quantitation of radioactivity used in a variety of biochemical experiments. The quantitative and high resolution capabilities of this instrument will allow performance of biochemical experiments not currently possible. The primary advantages of this technology over conventional autoradiography using X-ray film are an increased linear response, dynamic range, and sensitivity, as well as reduced exposure time. The instrument provides quantitative data from experiments involving the use of radioisotopes such as 32p, 35S, 14C, and 1251. An instrument of this type is not currently available in our building (Koshland Hall) at the University of California at Berkeley. The quantitation of radioactive samples will be carried out by the user groups in a variety of biochemical and molecular biology experiments detailed in Section 8. The primary areas of quantitative analysis will be nucleic acid-protein (DNA and RNA) interactions and signal transduction (GTPase activities of signalling proteins). The technology involves the use of storage phosphor screens which store energy from sample beta particle emission. The stored energy is then released by scanning the storage screen with a laser beam. This emission is detected by the instrument and stored on a computer hard disk. The data can then be accessed and analyzed using a software package provided with the instrument and transmitted through an ethernet network to user computer terminals. A number of quantitative biochemical experiments not currently possible will be feasible because the Molecular Dynamics Phosphorimager is vastly superior to X-ray film autoradiography.