A CHNS Analyzer to Investigate Resource Use and Availability In Biotic Systems

该提案涉及购买分子动力学425 SI的请求 磷光成像仪，一种用于检测和定量放射性的设备，用于各种生化实验。该仪器的定量和高分辨率能力将允许进行目前不可能的生化实验。该技术相对于使用X射线胶片的传统放射自显影的主要优点是增加了线性响应、动态范围和灵敏度，以及减少了曝光时间。该仪器提供了涉及使用放射性同位素如32 p，35 S，14 C和1251的实验的定量数据。这种类型的仪器目前在我们位于伯克利的加州大学的大楼（Koshland Hall）中不可用。 放射性样本的定量将由用户组在第8节详述的各种生化和分子生物学实验中进行。定量分析的主要领域是核酸-蛋白质（DNA和RNA）相互作用和信号转导（信号蛋白的GTdR活性）。该技术涉及使用存储荧光屏，其存储来自样品β粒子发射的能量。然后通过用激光束扫描存储屏来释放存储的能量。 该发射由仪器检测并存储在计算机硬盘上。然后可以使用仪器提供的软件包访问和分析数据，并通过以太网传输到用户计算机终端。由于分子动力学磷光成像仪大大上级X射线胶片放射自显影术，许多目前不可能的定量生化实验将是可行的。

This proposal involves a request to purchase the Molecular Dynamics 425SI Phosphorimager, a device for the detection and quantitation of radioactivity used in a variety of biochemical experiments. The quantitative and high resolution capabilities of this instrument will allow performance of biochemical experiments not currently possible. The primary advantages of this technology over conventional autoradiography using X-ray film are an increased linear response, dynamic range, and sensitivity, as well as reduced exposure time. The instrument provides quantitative data from experiments involving the use of radioisotopes such as 32p, 35S, 14C, and 1251. An instrument of this type is not currently available in our building (Koshland Hall) at the University of California at Berkeley. The quantitation of radioactive samples will be carried out by the user groups in a variety of biochemical and molecular biology experiments detailed in Section 8. The primary areas of quantitative analysis will be nucleic acid-protein (DNA and RNA) interactions and signal transduction (GTPase activities of signalling proteins). The technology involves the use of storage phosphor screens which store energy from sample beta particle emission. The stored energy is then released by scanning the storage screen with a laser beam. This emission is detected by the instrument and stored on a computer hard disk. The data can then be accessed and analyzed using a software package provided with the instrument and transmitted through an ethernet network to user computer terminals. A number of quantitative biochemical experiments not currently possible will be feasible because the Molecular Dynamics Phosphorimager is vastly superior to X-ray film autoradiography.