Purchase of a Reflectron Maldi-Tof Mass Spectrometer

化学研究仪器和设施计划的这一奖项将有助于为威斯康星州-麦迪逊大学化学系购买反射矩阵辅助激光解吸/电离（MALDI）飞行时间（TOF）质谱仪。 研究内容包括：（1）蛋白质设计与蛋白质重折叠;（2）寡聚肽和糖肽的MALDI-TOF质谱分析;（3）高分子碳水化合物抑制剂;（4）MALDI-TOF质谱在基因测序中的应用;（5）肽的反射MALDI-TOF质谱分析;（6）聚合物界面和动力学;和（7）乙烯基聚合物在A/W界面和星星聚合物在甲苯/水界面的横向扩散。 质谱（MS）是一种用于探测紧密结构细节并获得大量有机、生物有机和有机金属分子的分子组成的技术。 电喷雾技术的加入将质谱的范围扩展到蛋白质和核酸分子量，远远超过任何其他技术。 电喷雾电离与高分辨率结合使用提供了质谱分析中可用的最新技术。 它为化学家提供了表征化合物的最强大的工具之一。 在质谱分析中获得这种能力对于许多化学领域的前沿研究至关重要。

This award from the Chemistry Research Instrumentation and Facilities Program will aid in purchasing a Reflectron Matrix-Assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer for the Department of Chemistry at the University of Wisconsin- Madison. The research studies to be performed include the following: (1) Protein Design and Protein Refolding; (2) Analysis of Oligosaccharides and Glycopeptides with a MALDI-TOF Mass Spectrometer; (3) Polymeric Carbohydrate Inhibitors; (4) Applications of MALDI- TOF Mass Spectrometry in Gene Sequencing; (5) Analysis of Peptides with a Reflectron MALDI-TOF Mass Spectrometer; (6) Polymer Interfaces and Dynamics; and (7) Lateral Diffusion of Vinyl Polymers at the A/W Interface and of Star Polymer at the Toluene/Water Interface. Mass Spectrometry (MS) is a technique used to probe intimate structural details and to obtain the molecular compositions of a vast array of organic, bioorganic and organometallic molecules. The addition of the technique of electrospray extends the range of MS to protein and nucleic acid molecular weights far beyond any other technique. The use of electrospray ionization in combination with high resolution provides the latest techniques available in mass spectrometry. It affords the chemist one of the most powerful tools available for the characterization of compounds. The acquisition of this capability in mass spectrometry is essential for the prosecution of frontier research in many fields of chemistry.