PEET: Monographic and Phylogenetic Investigation of the Fossombroniineae (Hepaticophyta)

小行星9521883 认识到有必要加强在研究苔类（植物界苔类）的分类学专业知识。南伊利诺伊大学的雷蒙德·斯托勒和芭芭拉·克兰德尔·斯托勒建议通过参与一项关于梅茨geriales的Fossombroniinae亚目的“简单叶状体”分类群的世界性专题研究来让学生们学徒。 一个广泛的传统和现代研究计划将集中在这一分类学框架内预期出现的几百个物种上，包括：（1）用计算机成像系统进行形态测量分析，以评估这些小型、通常为叶生植物的配子体和孢子体特征的变异性;（2）蛋白质的淀粉凝胶电泳，以测量几个物种种群内和种群间的遗传变异水平;（3）孢子库研究，以确定自然界中共存的物种组合，并测量这些共存分类群之间的遗传差异;（4）叶绿体和核基因的DNA测序，与加州大学的布伦特·米什勒教授合作，以测量分类群之间的突变差异并构建突变基因;（5）在美国包括波多黎各的实地工作;和（6）建立一个万维网链接到该机构维护的肝病学计算机数据库。 对研究生和本科生的培训将把大学和波多黎各热带地区的联合收割机正式课程与实验室和博物馆研究结合起来，包括在加州大学伯克利分校的米什勒实验室的研究。 各种数据收集和数据分析工作的计算机化在整个项目的五年中得到充分整合，并将促进这些苔藓状植物信息的迅速传播。

9521883 Stotler Recognizing the need for enhancing taxonomic expertise in the study of liverworts (Division Hepaticophyta of the Kingdom Plantae) Profs. Raymond Stotler and Barbara Crandall-Stotler of Southern Illinois University propose to apprentice students through involvement in a worldwide monograph of the "simple thalloid" taxa of the suborder Fossombroniinae of the Metzgeriales. A broad program of traditional and modern studies will focus on the several hundred species expected to occur within this taxonomic framework, including: (1) morphometric analyses with computer imaging systems to assess variability in gametophytic and sporophytic features of these small, often epiphyllous plants; (2) starch gel electrophoresis of proteins to measure levels of genetic variability within and among populations of several species; (3) spore bank studies to determine coexisting species assemblages in nature, and to measure genetic differences between these co-occurring taxa; (4) DNA sequencing of chloroplast and nuclear genes, in cooperation with Prof. Brent Mishler at the University of California-@erkeley, to measure mutational differences between taxa and construct phylogenies; (5) field work in the U.S. including Puerto Rico; and (6) construction of a WorldWide Web link to computer databases on hepatics maintained at the institution. Training of graduate and undergraduate students will combine formal course work at the University and in tropical sites in Puerto Rico with laboratory and museum studies, including studies in Mishler's lab at UC-Berkeley. Computerization of various data-gathering and data-analyzing tasks is fully integrated throughout the five years of the project, and will enhance the prompt dissemination of information on these moss-like plants.