Flow and Separation in a Centrifugal Spectrometer

本研究是基于一种离心光谱方法的发展，该方法可以从混合物中分离出任何大小的颗粒并根据沉降速度进行分类。该概念适用于从技术中使用的较大的塑料珠到生物处理中遇到的微小蛋白质和病毒因子的颗粒的分离和分馏。该计划的最后阶段将包括三管齐下的方法：1。在国家科学基金会的支持下，对离心光谱仪的基本流动特性进行了实验研究。该设备的分选效率也将得到确立。2. 开发一个广泛适用的数值程序，用于精确模拟离心机内任何圆盘堆中混合物的非线性、轴对称、随时间变化的运动。3. 理论分析了水流、泥沙和边界层不稳定性及其对分离过程的影响。这项基础研究将为应用广泛的超离心机的工业化发展奠定基础。

ABSTRACT Harvey Greenspan CTS-9522703 MIT This research is based on the development of a method of centrifugal spectroscopy by which particles of any size can be separated from a mixture and sorted according to sedimentation velocity. The concept applies to the separation and fractionation of particles across the size spectrum from the larger plastic beads used in technology to the minute proteins and viral factors used encountered in bioprocessing. This final phase of the program will encompass a three pronged approach: 1. An experiment to examine the basic flow properties of the centrifugal spectrometer that was developed with prior NSF support. The sorting efficiency of the device will also be established. 2. Development of a broadly applicable numerical program for the accurate simulation of the non-linear, axisymmetric, time-dependent motion of a mixture in any disk stack in the centrifuge. 3. Theoretical analysis of the flow, sediment and boundary layer instabilities and their effect on the separation process. This fundamental research will set the stage for industrial development of an ultra centrifuge with varied and wide spread applications.