SGER: Montitoring Biological Processes in Single Living Cells

建议编号：IBN-9528526首席研究员：理查德·N·细胞通过细胞内信号通路的变化来响应环境和发育刺激，从而导致基因表达模式的改变。当这些过程发生在活细胞中时，监测它们是生物学研究的主要挑战。戴博士和他的同事们建议使用合成和分泌促性腺激素释放激素(GnRH)的神经细胞系作为模型系统，开发一种组合方法，以时间分辨率同时监测单个活细胞的基因表达、细胞内钙和电活动。这些研究适合用于探索性研究的小额赠款，因为建立从单个细胞确定所有这些细胞参数的技术相关的高风险，以及这些研究对生物学研究的重大影响。如果这些实验成功，人们将对细胞将信号从细胞表面传递到细胞核的机制有了新的了解。这项研究的另一个影响是，如果成功，用于这些研究的方法将适用于几乎任何提供膜和核事件独特时间联系的真核细胞系统的研究。将这项技术转移到生物体水平将使这位研究人员能够在不损害细胞完整性的情况下检查自然环境中的细胞，但允许他们明确地识别。

Proposal Number: IBN-9528526 Principal Investigator: Day, Richard N. Cells respond to environmental and developmental stimuli through changes in intracellular signaling pathways that lead to an altered pattern of gene expression. Monitoring of these processes as they occur in living cells is a major challenge for biological research. Dr. Day and his colleagues propose to use a neuronal cell line which synthesize and secrete gonadotropin-releasing hormone (GnRH) as a model system to develop a combined approach to simultaneously monitor gene expression, intracellular calcium and electrical activity in single living cells with temporal resolution. These studies are appropriate for a Small Grant for Exploratory Research because of the high risk associated with establishing the technology to ascertain all of these cellular parameters from a single cell and the significant impact of these studies to biological research. If these experiments are successful, new understanding will be forthcoming about the mechanisms used by cells to transmit signals from the cell surface to the nucleus. An additional impact of this research will be, if successful, the method used for these studies will be applicable to the study of virtually any eukaryotic cell system providing unique temporal linkage of membrane and nuclear events. The tpansfer of this technology to the organismal level will permit this investigator to examine cells in their natural surrounding without compromising their integrity, but permitting their unambiguous identification.