SGER: Montitoring Biological Processes in Single Living Cells

SGER：监测单个活细胞的生物过程

• 批准号: 9528526
• 负责人: Richard Day
• 金额: $ 5万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9528526&HistoricalAwards=false
• 关键词: SGER; Montitoring; Biological; Processes; Single

Proposal Number: IBN-9528526 Principal Investigator: Day, Richard N. Cells respond to environmental and developmental stimuli through changes in intracellular signaling pathways that lead to an altered pattern of gene expression. Monitoring of these processes as they occur in living cells is a major challenge for biological research. Dr. Day and his colleagues propose to use a neuronal cell line which synthesize and secrete gonadotropin-releasing hormone (GnRH) as a model system to develop a combined approach to simultaneously monitor gene expression, intracellular calcium and electrical activity in single living cells with temporal resolution. These studies are appropriate for a Small Grant for Exploratory Research because of the high risk associated with establishing the technology to ascertain all of these cellular parameters from a single cell and the significant impact of these studies to biological research. If these experiments are successful, new understanding will be forthcoming about the mechanisms used by cells to transmit signals from the cell surface to the nucleus. An additional impact of this research will be, if successful, the method used for these studies will be applicable to the study of virtually any eukaryotic cell system providing unique temporal linkage of membrane and nuclear events. The tpansfer of this technology to the organismal level will permit this investigator to examine cells in their natural surrounding without compromising their integrity, but permitting their unambiguous identification.

提案编号：IBN-9528526 首席研究员：Day，Richard N. 细胞通过细胞内信号传导途径的变化来响应环境和发育刺激，从而导致基因表达模式的改变。 监测活细胞中发生的这些过程是生物学研究的主要挑战。 Day 博士和他的同事建议使用合成和分泌促性腺激素释放激素 (GnRH) 的神经元细胞系作为模型系统，开发一种组合方法，以时间分辨率同时监测单个活细胞中的基因表达、细胞内钙和电活动。 这些研究适合探索性研究的小额资助，因为建立确定单个细胞的所有这些细胞参数的技术具有很高的风险，并且这些研究对生物学研究具有重大影响。 如果这些实验成功，将对细胞将信号从细胞表面传输到细胞核的机制产生新的理解。 这项研究的另一个影响是，如果成功，这些研究所用的方法将适用于几乎任何真核细胞系统的研究，提供膜和核事件的独特时间联系。 这项技术转移到生物体水平将使研究人员能够检查自然环境中的细胞，而不会损害其完整性，但可以明确识别它们。