International Research Fellow Awards Program: Identification of AFLP Markers Linked to Disease Resistance Genes in the Commercial Strawberry

国际研究员奖励计划：鉴定与商业草莓抗病基因相关的 AFLP 标记

• 批准号: 9600487
• 负责人: Kenneth Haymes
• 金额: $ 4.29万
• 依托单位: Fellowships
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9600487&HistoricalAwards=false
• 关键词: International; Research; Fellow; Awards; Program

9600487 Haymes The International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twentyfour months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit of Dr. Kenneth M. Haymes to work with Dr. ir A. M. P. den Nijs at the Centre for Plant Breeding and Reproductive Research (CPRO-DLO) in Wageningen, Netherlands. Dr. Haymes and Dr. den Nijs will use molecular techniques to obtain markers for regions of the strawberry genome that are linked to genes Pfr1, Pfr2 and Pfr6, which confer resistance to the pathogenic fungus Phytophthora fragariae Hickman (red stele root rot). They will also look at existing strawberry stocks for the presence of these markers to develop a rational breeding program for improved resistance to Phytophthora infection. A powerful mapping technique, amplified fragment length polymorphism (AFLP) will be used following bulked segregant analysis to attempt to isolate markers closely linked to the Pfr1 marker. Bulked segregant analysis and AFLP will then be applied to obtain markers near to the other resistance genes. The commercial strawberry is octaploid and thus molecular markers are the best hope for accomplishing rational linkage studies of disease resistance and other traits. Once they obtain markers that are tightly linked to genes which increase resistance to fungal infection, plant stocks will be examined for the purpose of identifying appropriate breeding stocks to produce strawberry plants containing multiple resistance genes. ***

小行星9600487 国际研究员奖励计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖项提供了联合研究的机会，以及使用国外独特或互补的设施，专业知识和实验条件。 该奖项将支持肯尼斯·M博士为期12个月的博士后研究访问。Haymes与Dr. ir A. M. P. den Nijs在荷兰瓦赫宁根的植物育种和繁殖研究中心（CPRO-DLO）。 Haymes博士和den Nijs博士将利用分子技术获得草莓基因组中与基因Pfr 1、Pfr 2和Pfr 6相关的区域的标记，这些基因赋予对致病真菌草莓疫霉Hickman（红色中柱根腐病）的抗性。 他们还将研究现有的草莓库存是否存在这些标记，以制定合理的育种计划，提高对疫霉感染的抗性。 一个强大的作图技术，扩增片段长度多态性（AFLP）将使用以下散装分离分析，试图分离标记密切连锁的Pfr 1标记。 然后进行群体分离分析和AFLP分析，以获得与其他抗性基因接近的标记。 商品草莓为八倍体，利用分子标记进行抗病性等性状的合理连锁研究是最有希望的。 一旦他们获得了与增加对真菌感染的抗性的基因紧密相连的标记，将对植物种群进行检查，以确定合适的育种种群，从而生产出含有多种抗性基因的草莓植物。 ***