Molecular and Genetic Characterization of a Class II Flagellar Operon in Bacillus subtilis

枯草芽孢杆菌 II 类鞭毛操纵子的分子和遗传特征

• 批准号: 9600932
• 负责人: Leticia Marquez-Magana
• 金额: $ 49.86万
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9600932&HistoricalAwards=false
• 关键词: Molecular; Genetic; Characterization; Class; II

Marquez-Magana 96-00932 The overall objective of this Career Development Plan is to create a balanced (50/50) effort between research and teaching. In this way the principal investigator (P.I.) intends to serve the community as a role model for students considering a career in science. The proposed research activities focus on the molecular and genetic characterization of the fla/che operon, a large flagellar transcription unit in Bacillus subtilis, and the regulation of its expression. Although much of the DNA that makes up the fla/che transcription unit has been cloned, sequenced, and characterized genetically, the promoter element(s) that initiates its transcription has not been identified. Additionally , the 3' end of the operon has not been characterized. Molecular and genetic analysis of the fla/che operon will not only increase our knowledge of flagellar gene expression in B. subtilis, but will contribute to our understanding of flagellar regulons in many different organisms where homologous regulons appear to exist. Part of these research activities have been incorporated into a course that the a P.I. has developed. As a faculty member, the goal of the P.I. is to foster the excitement of scientific discovery in undergraduate and graduate students at San Francisco State University (SFSU), and to empower them with the tools to pursue a successful career in science. The specific objectives are to: Utilize microbial genetic techniques for the molecular analysis of the fla/che operon of Bacillus subtilis: Characterize transcription initiation sites Clone and study the 3' end of operon Screen for and analyze regulatory mutants of the fla/che operon Empower undergraduate and graduate students with the knowledge and technology of molecular genetic approaches: Introduce undergraduate students to the power of genetic engineering Train graduate students in microbial genetics methodology 3. Recruit and mentor minority undergraduate and graduate students in the fields of microbial genetics and molecular biology: Participate in institutional and community programs that reach out to minority students Mentor minority students undertaking independent research These objectives are firmly supported by both the Department of Biology and tip-level administrators at San Francisco State University. Faculty in the Department of Biology believe strongly that an active research program provides students with the opportunity to gain first hand experience in how science operates and gives them skills not attainable in a traditional classroom setting. Additionally, the university at large is committed to the eduation of the ethnically diverse Bay Area population.

Marquez-Magana 96-00932这项职业发展计划的总体目标是在研究和教学之间创造平衡（50/50）的工作。 这样，首席研究员（P.I.）打算将社区作为考虑科学职业的学生的榜样。 提出的研究活动集中于FLA/CHE操纵子的分子和遗传表征，枯草芽孢杆菌中的大型鞭毛转录单元及其表达的调节。 尽管构成FLA/CHE转录单元的大部分DNA已被克隆，测序和遗传表征，但尚未鉴定出启动其转录的启动子元素。 此外，尚未表征操纵子的3'末端。 FLA/CHE操纵子的分子和遗传分析不仅会增加我们对枯草芽孢杆菌中鞭毛基因表达的了解，而且还将有助于我们对似乎存在同源调节的许多不同生物中的鞭毛调节。 这些研究活动的一部分已纳入了A P.I.已经发展了。 作为教职员工，P.I.的目标是为了促进旧金山州立大学（SFSU）的本科生和研究生的科学发现的兴奋，并通过从事科学领域成功职业的工具增强他们的能力。 具体目标是：利用微生物遗传技术来对枯草芽孢杆菌的FLA/CHE操纵子进行分子分析：特征转录起始站点克隆并研究操纵子屏幕的3'结束，以进行操纵子屏幕的3'结束，并分析FLA/CHE Operon的调节性突变体，以使FLA/CHE Operon的能力赋予学生的知识和研究范围，从而介绍了遗传学的学生：微生物遗传学方法论3。在微生物遗传学和分子生物学领域招募和导师的少数群体本科生和研究生：参与与少数群体指导的少数群体指导的机构和社区计划，从事独立研究的独立研究，这些目标是由生物学和小费级别级别级别委员会在San Francisco的国家支持的。 生物学系的教师坚信，一项活跃的研究计划为学生提供了在科学运作方式上获得第一手经验并为他们提供在传统教室环境中无法获得的技能的机会。 此外，整个大学致力于教授种族多样化的湾区人口。