Molecular and Genetic Characterization of a Class II Flagellar Operon in Bacillus subtilis

枯草芽孢杆菌 II 类鞭毛操纵子的分子和遗传特征

• 批准号: 9600932
• 负责人: Leticia Marquez-Magana
• 金额: $ 49.86万
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9600932&HistoricalAwards=false
• 关键词: Molecular; Genetic; Characterization; Class; II

Marquez-Magana 96-00932 The overall objective of this Career Development Plan is to create a balanced (50/50) effort between research and teaching. In this way the principal investigator (P.I.) intends to serve the community as a role model for students considering a career in science. The proposed research activities focus on the molecular and genetic characterization of the fla/che operon, a large flagellar transcription unit in Bacillus subtilis, and the regulation of its expression. Although much of the DNA that makes up the fla/che transcription unit has been cloned, sequenced, and characterized genetically, the promoter element(s) that initiates its transcription has not been identified. Additionally , the 3' end of the operon has not been characterized. Molecular and genetic analysis of the fla/che operon will not only increase our knowledge of flagellar gene expression in B. subtilis, but will contribute to our understanding of flagellar regulons in many different organisms where homologous regulons appear to exist. Part of these research activities have been incorporated into a course that the a P.I. has developed. As a faculty member, the goal of the P.I. is to foster the excitement of scientific discovery in undergraduate and graduate students at San Francisco State University (SFSU), and to empower them with the tools to pursue a successful career in science. The specific objectives are to: Utilize microbial genetic techniques for the molecular analysis of the fla/che operon of Bacillus subtilis: Characterize transcription initiation sites Clone and study the 3' end of operon Screen for and analyze regulatory mutants of the fla/che operon Empower undergraduate and graduate students with the knowledge and technology of molecular genetic approaches: Introduce undergraduate students to the power of genetic engineering Train graduate students in microbial genetics methodology 3. Recruit and mentor minority undergraduate and graduate students in the fields of microbial genetics and molecular biology: Participate in institutional and community programs that reach out to minority students Mentor minority students undertaking independent research These objectives are firmly supported by both the Department of Biology and tip-level administrators at San Francisco State University. Faculty in the Department of Biology believe strongly that an active research program provides students with the opportunity to gain first hand experience in how science operates and gives them skills not attainable in a traditional classroom setting. Additionally, the university at large is committed to the eduation of the ethnically diverse Bay Area population.

马奎兹-马格纳96-00932这个职业发展计划的总体目标是在研究和教学之间创造一个平衡的努力(50/50)。这样，首席调查员(P.I.)打算为社区服务，为考虑从事科学事业的学生树立榜样。拟开展的研究活动集中在枯草芽孢杆菌中大型鞭毛转录单位Fla/ChE操纵子的分子和遗传特征及其表达调控方面。尽管组成FLA/CHE转录单位的大部分DNA已经被克隆、测序和鉴定，但启动其转录的启动子元件(S)尚未被鉴定。此外，操纵子的3‘端还没有被表征。对Fla/ChE操纵子的分子和遗传分析不仅将增加我们对枯草杆菌鞭毛基因表达的了解，而且将有助于我们理解许多不同生物中存在同源调控子的鞭毛调控子。这些研究活动中的一部分已经被纳入了AI开发的一门课程。作为一名教职员工，P.I.的目标是在旧金山州立大学(SFSU)培养本科生和研究生对科学发现的兴奋，并赋予他们在科学领域追求成功职业生涯的工具。具体目标是：利用微生物遗传技术对枯草芽孢杆菌的Fla/ChE操纵子进行分子分析：鉴定转录起始点克隆，研究Fla/ChE操纵子的3‘端调控突变体，并分析Fla/ChE操纵子的调控突变：向本科生和研究生介绍分子遗传学方法的知识和技术：向本科生介绍基因工程的力量微生物遗传学方法论培训研究生3.招聘和指导该领域的少数本科生和研究生微生物遗传学和分子生物学：参与机构和社区项目，接触少数族裔学生，指导少数族裔学生进行独立研究，这些目标得到了旧金山州立大学生物系和顶层管理人员的坚定支持。生物系的教职员工坚信，积极的研究项目为学生提供了获得科学如何运作的第一手经验的机会，并赋予他们在传统课堂环境中无法获得的技能。此外，该大学致力于对湾区不同种族的人口进行教育。