Integrated Computer Crystallization Laboratories

加州大学旧金山分校生物化学系有一个充满活力的晶体学实验室。过去几年的成功鼓励了该大学扩大结构生物学的教员任命。1995年，生物科学项目研究员布丽吉特·劳曼（Brigitte Raumann）作为独立研究员加入。1996年，Wendell Lim和Jonathan Weissman加入，担任细胞和分子药理学助理教授。这种扩张，以及在生物化学结构实验室工作的研究生和博士后数量的自然增长，几乎使加州大学旧金山分校的x射线衍射和计算机设备不堪重负。在这个提案中，我们提出了增加生物化学系三维结构生物学实验设施的计划，并在细胞和分子药理学系内装备一个新的实验室，配备x射线衍射设备和计算机。拟议中的新设备和更新的实验室，一个是细胞和分子药理学，一个是生物化学，将在其使用和结构上，平等地向UCSF的所有x射线晶体学家开放。一个由三层组成的庞大的计算机系统计划允许以透明和同等的方式进行计算。在下面的叙述中，我们列出了六位主要研究人员的项目，并说明了对新的公共设备的需求。细胞和分子药理学系用来容纳新机器的实验室将进行翻新：将增加一堵墙，将核心计算机与x射线设备分开，更换管道，安装电气装置，并建造一个安全外壳。这些项目大部分是由NIH资助给私家侦探的，以叙述的形式简要描述，并尽可能参考已发表的作品，然后是一个定义项目和人员的表格。它按标题列出了各个项目，从事该项目的主要科学家和项目的状态。该状态提供了当前数据收集需求的简要总结。

UCSF has had a vibrant crystallography lab in the Department of Biochemistry. The successes over the last years encouraged the university to expand the Faculty Appointments in Structural Biology. In 1995, Brigitte Raumann, a Program in Biological Sciences Fellow, joined as an independent researcher. In 1996, Wendell Lim and Jonathan Weissman joined as Assistant Professors in Cellular and Molecular Pharmacology. This expansion, and the natural growth in numbers of graduate students and postdoctoral fellows working the structural labs in Biochemistry, has virtually overwhelmed the X-ray diffraction and computer facilities at UCSF. In this proposal we present plans to augment the facilities for three dimensional experimental Structural Biology for the Department of Biochemistry and to equip a new lab with X-ray diffraction equipment and computers within the Department of Cellular and Molecular Pharmacology. The proposed newly equipped and updated labs, one in Cellular and Molecular Pharmacology and one in Biochemistry, are to be in their use and structure, equally accessible to all X-ray crystallographers at UCSF. An extensive computer system built in three tiers is planned to permit computing also in transparent and equivalent ways. In the following narrative, we list the projects of the six principal investigators and demonstrate the need for the new communal equipment. The laboratory to house the new machines in the Department of Cellular and Molecular Pharmacology will be renovated: a wall is to be added for separating the core computers from the X-ray set, plumbing changed, electrical installation and a safety enclosure will be huilt. The projects, most supported by NIH grants to the P.I.s, are briefly described in narrative form, with references to published work where possible, and followed by a table defining projects and personnel. This lists the individual projects by title, the principal scientists working on the project and the status of the project. T he status provides a brief summary of the current needs for data collection.