Stimulus-Secretion Coupling in Mucosal Mast Cells: Studies at the Single-Cell Level

粘膜肥大细胞的刺激-分泌耦合：单细胞水平的研究

• 批准号: 9604432
• 负责人: Clare Fewtrell
• 金额: --
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604432&HistoricalAwards=false
• 关键词: Stimulus; Secretion; Coupling; Mucosal; Mast

Abstract 9604432 Fewtrell The role of calcium in stimulus-response coupling in non-excitable cells is not well understood. The calcium responses of individual cells within a population are extremely diverse, often displaying marked oscillations in calcium, however little is known about the specific features of the calcium signal that are important for the functional response of the cell. In these studies the temporal relationship between changes in intracellular calcium and secretion will be studied at the single cell level in non-excitable cells, using a mucosal mast cell line as a model. The calcium responses of these cells have been well characterized and the exocytosis of secretory granules containing histamine, serotonin and other mediators by indo-1 photometry, and the secretion of serotonin containing granules will be monitored simultaneously in the same cell by constant potential amperometry. Thus the calcium response associated with a single exocytotic events or burst of secretion can be measured. Fluorescence imagining will monitor the calcium responses of a large number of individual cells simultaneously. The hypothesis that an increase in intracellular calcium is essential for exocytosis will be tested using a variety of stimuli to mast cell lines, cell clones and transfected cell lines. The requirement for protein kinase C activation and its role in modulating the secretory response will be re-examined at the single cell level. Information obtained about calcium signaling in mast cells will contribute to the overall understanding of the role and regulation of this important signaling molecule in many different cell types and systems. ***

摘要9604432 Fewtrell钙在非兴奋性细胞的刺激-反应耦合中的作用尚不清楚。一个群体中单个细胞的钙反应是非常不同的，经常表现出明显的钙振荡，然而人们对钙信号的具体特征知之甚少，这些特征对于细胞的功能反应是重要的。在这些研究中，将以粘膜肥大细胞系为模型，在单细胞水平上研究非兴奋性细胞内钙离子变化和分泌之间的时间关系。这些细胞的钙反应已被很好地表征，含组胺、5-羟色胺等介质的分泌颗粒的胞吐和含5-羟色胺的分泌颗粒的分泌将在同一细胞内用恒电位安培法同时监测。因此，可以测量与单个胞吐事件或分泌爆发相关的钙反应。荧光成像将同时监测大量单个细胞的钙反应。细胞内钙的增加对胞吐是必不可少的这一假设将被用各种刺激来测试肥大细胞系、细胞克隆和转基因细胞系。对蛋白激酶C激活的要求及其在调节分泌反应中的作用将在单细胞水平上重新审查。获得有关肥大细胞钙信号的信息将有助于全面了解这一重要信号分子在许多不同细胞类型和系统中的作用和调控。***