Characterization of C3 Phosphoenolpyruvate Carboxylase Isoforms that Operate in an Inducible C4-Type PhotosyntheticSystem

在诱导型 C4 型光合系统中运行的 C3 磷酸烯醇丙酮酸羧化酶亚型的表征

• 批准号: 9604518
• 负责人: George Bowes
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604518&HistoricalAwards=false
• 关键词: Characterization; C3; Phosphoenolpyruvate; Carboxylase; Isoforms

9604518 Bowes Dr. Bowes has worked on the mechanisms of carbon fixation during photosynthesis for many years. His work has recently moved into a new area, and the laboratory is now using molecular analysis of gene expression in addition to biochemical analyses. Adding to the three basic types of carbon fixation mechanisms, Dr. Bowes now adds another one. His group detected plants which show inducible aspects of C4 metabolism without expressing the morphological/anatomical characteristics of this pathway. The work proposed promises to provide an integration of molecular, evolutionary and physiological aspects of the new pathway. The group will isolate the transcripts for key enzymes of the pathway, place them into an evolutionary context and study the biochemical characteristics of these enzymes. The focus is on the differences in activity regulation that can be expected to distinguish the enzymes that are expressed when the plants are in different fixation modes.

9604518鲍斯博士多年来一直致力于光合作用中碳固定机制的研究。他的工作最近进入了一个新的领域，除了生化分析外，该实验室现在还使用基因表达的分子分析。除了三种基本的碳固定机制外，鲍斯博士现在又增加了一种。他的团队检测到了一些植物，这些植物表现出C4代谢的可诱导方面，但没有表现出这一途径的形态/解剖学特征。这项提出的工作有望提供新途径的分子、进化和生理方面的整合。该小组将分离该途径的关键酶的转录本，将它们置于进化的背景下，并研究这些酶的生化特征。重点放在活性调节的差异上，这些差异可以预期区分植物在不同固定模式下表达的酶。