Structural aspects of membrane fusion investigated by X-ray diffraction and imaging (B01)

该项目的目标是研究融合茎的结构，特别强调使用不同的X射线技术重建融合蛋白质（如SNARE）的脂质膜。最终目标是揭示蛋白质在茎结构的三维电子密度中的位置。我们想要监测“结晶”转变附近秸秆的瞬时形成。最后，我们的目的是在一个详细的调查与不同的重组SNARE和突变体的囊泡之间的对接状态的结构。

The goal of this project is to study the structure of fusion stalks with particular emphasis on lipid membranes with reconstituted fusogenic proteins such as SNAREs, using different X-ray techniques. The ultimate goal is to reveal the positions of the proteins in the three-dimensional electron density of the stalk structure. We want to monitor the transient formation of stalks near the "crystallization" transition. Finally, we aim at a detailed investigation of the structure of the docking state between vesicles with different reconstituted SNAREs and mutants.