DISSERTATION RESEARCH: Molecular Phylogeny of the Ericaceae using RNA Polymerase II

论文研究：使用 RNA 聚合酶 II 进行杜鹃花科的分子系统发育

• 批准号: 9623643
• 负责人: Benjamin Hall
• 金额: $ 0.4万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-15 至 1998-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9623643&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Molecular; Phylogeny; Ericaceae

9623643 HALL Reconstruction of the historical (OphylogeneticO) relationships among plant groups, through comparative analysis of nuclear and organellar ribosomal DNA sequences, is an integral part of systematic biology. Researchers increasingly recognize the need to obtain data from several different DNA sequences, representing independent genetic loci, because a phylogeny inferred from any single gene may not accurately recover the relationships among the organisms under investigation. However, the number of genes known to be both useful for these investigations and accessible to researchers (quickly and inexpensively obtainable from a wide range of organisms) is still very small. In an effort to expand the pool of nuclear genes available for molecular systematic analyses at lower taxonomic levels, Amy Denton, under the supervision of Benjamin Hall, will evaluate the phylogenetic utility of sequences within the RNA polymerase II (RPB) gene. The researchers will first determine if, as expected, RPB gene sequences are useful for phylogenetic assessments of flowering plant taxa at the family level and below; they will compare phylogenies constructed from these sequences to phylogenies of the plant family Ericaceae (which includes rhododendrons, azaleas and blueberries) derived from other data. If these results confirm predictions, Denton and Hall will then use RPB gene sequences to investigate the complex, and poorly understood, phylogenetic relationships among species of the large, horticulturally valuable genus Rhododendron.

9623643霍尔通过对核糖体和细胞器核糖体DNA序列的比较分析，重建植物类群之间的历史(系统发育)关系，是系统生物学的组成部分。研究人员越来越认识到，需要从代表独立遗传位点的几个不同的DNA序列中获得数据，因为从任何单个基因推断的系统发育可能无法准确恢复被研究生物之间的关系。然而，已知对这些研究有用且研究人员可获得的基因数量仍然很少(可从广泛的生物体中快速且廉价地获得)。为了扩大可用于较低分类水平的分子系统分析的核基因库，艾米·丹顿将在本杰明·霍尔的监督下，评估RNA聚合酶II(RPB)基因内序列的系统发育效用。研究人员将首先确定RPB基因序列是否如预期的那样对科及科以下的开花植物分类群的系统发育评估有用；他们将把根据这些序列构建的系统发育图与从其他数据得出的杜鹃花科植物(包括杜鹃花、杜鹃花和蓝莓)的系统发育进行比较。如果这些结果证实了预测，丹顿和霍尔将使用rpb基因序列来研究大型、有园艺价值的杜鹃属物种之间复杂而鲜为人知的系统发育关系。