Chicken CR1 Elements: Prototypes of a Novel and Ancient Superfamily of Vertebrates Non-LTR Retrotransposons

鸡 CR1 元件：一种新颖且古老的脊椎动物非 LTR 反转录转座子超家族的原型

• 批准号: 9630227
• 负责人: John Burch
• 金额: $ 28.4万
• 依托单位: Institute For Cancer Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630227&HistoricalAwards=false
• 关键词: Chicken; CR1; Elements; Prototypes; Novel

Burch MCB-96-3022 Eukaryotic genomes contain interspersed repetitive sequence elements that derive from the reverse flow of genetic information. These retrotransposons can be of two classes based upon whether they contain or lack long terminal repeat (LTR) sequences similar to those found in retroviruses. The chicken repeat 1 (CR1) elements are non-LTR retrotransposons and encodes its own reverse transcriptase. Sequence homology to elements found in fish, frogs, reptiles and, perhaps, mammals suggest that these elements are the prototype for a superfamily of non-LTR retrotransposons that exist in multiple vertebrate classes. Full-length elements for two distinct subfamilies of CR1 elements will be isolated and characterized. It will be determined if the CR1 reverse transcriptase is functional and avian and non-avian cell cultures will be assayed for CR1 retrotransposition events. %%% Retrotransposons are a major source of mutations in lower eukaryotes such as yeast and Drosophila and are implicated in several human genetic disorders. They also contribute to genome instability in other ways, for example, as targets for non-reciprocal homologous recombination events. This work will contribute to insight into the mechanisms and sequences required for the transposition of an evolutionary successful non-LTR element, CR1.

Burch MCB-96-3022真核生物基因组含有源自遗传信息反向流动的散布重复序列元件。这些逆转录转座子可以分为两类，这取决于它们是否含有或缺乏类似于逆转录病毒中发现的长末端重复序列（LTR）。鸡重复序列1（CR 1）元件是非LTR反转录转座子，并编码其自身的逆转录酶。与在鱼、蛙、爬行动物和哺乳动物中发现的元件的序列同源性表明，这些元件是存在于多个脊椎动物类中的非LTR反转录转座子超家族的原型。CR 1元件的两个不同亚家族的全长元件将被分离和表征。将确定CR 1逆转录酶是否有功能，并将测定禽类和非禽类细胞培养物的CR 1逆转录转座事件。 逆转录转座子是低等真核生物（如酵母和果蝇）突变的主要来源，并与几种人类遗传疾病有关。它们还以其他方式促进基因组不稳定性，例如，作为非相互同源重组事件的靶标。这项工作将有助于深入了解一个进化成功的非LTR元件，CR 1的转座所需的机制和序列。