Role of NAC transcription factors in plant senescence

NAC家族的转录因子在衰老调控中发挥着重要作用，但其调控衰老的分子机制尚不清楚。目前的提案涉及对拟南芥转录因子ANAC047调节衰老的功能分析。与最近报道的NAC转录因子JUB1一样，ANAC047的表达被过氧化氢处理迅速刺激；然而，与JUB1相反，它正向调节衰老。ANAC047在发育和N饥饿诱导的衰老过程中都被诱导，因此是在For框架内进一步功能研究的一个特别有趣的候选基因。最近，我们发现了ANAC047的两个可能的下游靶点，即编码乙烯生物合成关键酶的ACC氧化酶5(ACO5)和编码衰老诱导的乙醇酸氧化酶的GOX3。拟开展的工作包括ANAC047-ACO5/GOX3衰老调控级联的功能分析以及ANAC047基因调控网络的进一步解码。其他工作旨在通过考虑在For的第一个资助期内获得的关于衰老调控转录因子的知识来修改N饥饿诱导的甘蓝型油菜衰老。该提议包括与FOR中的合作伙伴合作，建立更广泛的植物N控制衰老的生物计算询问或基因调控网络。关键词：拟南芥，油菜籽，NAC转录因子，衰老。

Transcription factors of the NAC family play an important role for the regulation of senescence, however, the molecular mechanisms through which they control senescence are only vaguely known. The current proposal addresses the functional analysis of the regulation of senescence by the Arabidopsis thaliana transcription factor ANAC047. Like the recently reported NAC transcription factor JUB1, expression of ANAC047 is rapidly stimulated by treatment with H2O2; however, in contrast to JUB1 it positively regulates senescence. ANAC047 is induced during both, developmental and N starvation-induced senescence and thus represents a particularly interesting candidate for further functional studies within the frame of the FOR. Recently, we discovered two likely downstream targets of ANAC047, i.e. ACC OXIDASE 5 (ACO5), which encodes a key enzyme of ethylene biosynthesis, and GOX3, which encodes a senescence-induced glycolate oxidase. The proposed work includes the functional analysis of the ANAC047 - ACO5/GOX3 regulatory cascade for senescence as well as the further decoding of the gene regulatory network of ANAC047. Additional work aims at modifying N starvation-induced senescence in Brassica napus by taking into account knowledge gained on senescence regulatory transcription factors during the first funding period of the FOR. The proposal includes a wider bio-computational interrogation or the gene regulatory networks of N-controlled senescence in plants, in collaboration with partners in the FOR.Keywords: Arabidopsis, rapeseed, NAC transcription factors, senescence.