Role of NAC transcription factors in plant senescence

NAC家族转录因子在衰老调控中发挥着重要作用，但其调控衰老的分子机制尚不清楚。目前的建议是通过拟南芥转录因子ANAC047进行衰老调控的功能分析。与最近报道的NAC转录因子JUB1一样，ANAC047的表达在H2O2处理下被迅速刺激；然而，与JUB1相反，它积极调节衰老。ANAC047在发育和氮饥饿诱导的衰老过程中都被诱导，因此在for的框架内代表了一个特别有趣的进一步功能研究的候选者。最近，我们发现了ANAC047的两个可能的下游靶点，即ACC氧化酶5 (ACO5)，它编码乙烯生物合成的关键酶，GOX3编码衰老诱导的乙醇酸氧化酶。研究内容包括ANAC047 - ACO5/GOX3衰老调控级联的功能分析，以及ANAC047基因调控网络的进一步解码。额外的工作旨在通过考虑在FOR的第一个资助期间获得的衰老调控转录因子的知识来修饰氮饥饿诱导的甘蓝型油菜衰老。该提案包括一个更广泛的生物计算讯问或植物n控制衰老的基因调控网络，与合作伙伴在FOR。关键词：拟南芥；油菜籽；NAC转录因子；

Transcription factors of the NAC family play an important role for the regulation of senescence, however, the molecular mechanisms through which they control senescence are only vaguely known. The current proposal addresses the functional analysis of the regulation of senescence by the Arabidopsis thaliana transcription factor ANAC047. Like the recently reported NAC transcription factor JUB1, expression of ANAC047 is rapidly stimulated by treatment with H2O2; however, in contrast to JUB1 it positively regulates senescence. ANAC047 is induced during both, developmental and N starvation-induced senescence and thus represents a particularly interesting candidate for further functional studies within the frame of the FOR. Recently, we discovered two likely downstream targets of ANAC047, i.e. ACC OXIDASE 5 (ACO5), which encodes a key enzyme of ethylene biosynthesis, and GOX3, which encodes a senescence-induced glycolate oxidase. The proposed work includes the functional analysis of the ANAC047 - ACO5/GOX3 regulatory cascade for senescence as well as the further decoding of the gene regulatory network of ANAC047. Additional work aims at modifying N starvation-induced senescence in Brassica napus by taking into account knowledge gained on senescence regulatory transcription factors during the first funding period of the FOR. The proposal includes a wider bio-computational interrogation or the gene regulatory networks of N-controlled senescence in plants, in collaboration with partners in the FOR.Keywords: Arabidopsis, rapeseed, NAC transcription factors, senescence.