Role of NAC transcription factors in plant senescence

NAC转录因子在植物衰老中的作用

• 批准号: 111000993
• 负责人: Professorin Dr. Salma Balazadeh
• 金额: --
• 依托单位: Universiteit Leiden
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/111000993?language=en
• 关键词: Role; NAC; transcription; factors; plant

Transcription factors of the NAC family play an important role for the regulation of senescence, however, the molecular mechanisms through which they control senescence are only vaguely known. The current proposal addresses the functional analysis of the regulation of senescence by the Arabidopsis thaliana transcription factor ANAC047. Like the recently reported NAC transcription factor JUB1, expression of ANAC047 is rapidly stimulated by treatment with H2O2; however, in contrast to JUB1 it positively regulates senescence. ANAC047 is induced during both, developmental and N starvation-induced senescence and thus represents a particularly interesting candidate for further functional studies within the frame of the FOR. Recently, we discovered two likely downstream targets of ANAC047, i.e. ACC OXIDASE 5 (ACO5), which encodes a key enzyme of ethylene biosynthesis, and GOX3, which encodes a senescence-induced glycolate oxidase. The proposed work includes the functional analysis of the ANAC047 - ACO5/GOX3 regulatory cascade for senescence as well as the further decoding of the gene regulatory network of ANAC047. Additional work aims at modifying N starvation-induced senescence in Brassica napus by taking into account knowledge gained on senescence regulatory transcription factors during the first funding period of the FOR. The proposal includes a wider bio-computational interrogation or the gene regulatory networks of N-controlled senescence in plants, in collaboration with partners in the FOR.Keywords: Arabidopsis, rapeseed, NAC transcription factors, senescence.

NAC家族的转录因子在衰老的调控中起着重要的作用，然而，它们控制衰老的分子机制却知之甚少。目前的建议解决了拟南芥转录因子ANAC 047的衰老调控的功能分析。与最近报道的NAC转录因子JUB 1一样，ANAC 047的表达通过用H2 O2处理而迅速刺激;然而，与JUB 1相反，它正调控衰老。ANAC 047在发育和N饥饿诱导的衰老过程中诱导，因此代表了FOR框架内进一步功能研究的特别有趣的候选者。最近，我们发现了ANAC 047的两个可能的下游靶点，即ACC氧化酶5（ACOXIDASE 5，ACO 5），其编码乙烯生物合成的关键酶，和GOX 3，其编码衰老诱导的乙醇酸氧化酶。本论文的工作包括对衰老调控级联反应ANAC 047-ACO 5/GOX 3的功能分析，以及进一步解码ANAC 047的基因调控网络。其他工作的目的是修改N饥饿诱导的甘蓝型油菜衰老，考虑到知识的衰老调控转录因子在第一个资助期的FOR。该提案包括一个更广泛的生物计算的审讯或N-控制的植物衰老的基因调控网络，与合作伙伴在FOR.Keywords：拟南芥，油菜籽，NAC转录因子，衰老。