The Use of Modern Molecular Techniques to Foster Collaborative Learning and to Enhance Individual Undergraduate Research

利用现代分子技术促进协作学习并加强本科生个人研究

• 批准号: 9651403
• 负责人: Sharon Rose
• 金额: $ 2.02万
• 依托单位: Willamette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9651403&HistoricalAwards=false
• 关键词: Use; Modern; Molecular; Techniques; Foster

Because of the large student response to the Molecular Genetics and Microbial Ecology courses in the Biology curriculum, this project designed a collaborative project centered around the theory of the two courses and the combined expertise of faculty and students. The pilot project included the isolation and purification of coliphages from sewage followed by enzyme restriction analyses of coliphage DNA. Newly purchased instruments now enhance and expand this project to include the isolation, restriction mapping, and polymerase chain reaction amplification of genomic DNA. Students are also able to conduct qualitative analyses with the acquisition of DNA sequencing capabilities and quantitative analyses using an optical densitometer. With the new equipment, students are able to conduct modern techniques including PCR amplification of DNA, Southern blotting, probe hybridization, DNA sequencing, and chemiluminescent probing. These capabilities can greatly increase students' understanding of genetic theory and phylogeny and can also enable students to learn how to generate molecular markers to be used in genetic and microbial investigations. A knowledge of these techniques is essential to the success of students in their research projects, in their future studies, and in an increasingly technical world.

由于学生对生物学课程中的分子遗传学和微生物生态学课程反应强烈，该项目设计了一个以两门课程的理论以及教师和学生的综合专业知识为中心的合作项目。该试点项目包括从污水中分离和纯化大肠杆菌噬菌体，然后对大肠杆菌噬菌体 DNA 进行酶切分析。新购买的仪器现在增强和扩展了该项目，包括基因组 DNA 的分离、限制性图谱和聚合酶链式反应扩增。学生还能够通过获得 DNA 测序功能进行定性分析，并使用光密度计进行定量分析。借助新设备，学生能够进行现代技术，包括 DNA PCR 扩增、DNA 印迹、探针杂交、DNA 测序和化学发光探测。这些能力可以极大地增加学生对遗传理论和系统发育学的理解，还可以使学生学习如何生成用于遗传和微生物研究的分子标记。了解这些技术对于学生在研究项目、未来的学习以及日益技术化的世界中取得成功至关重要。