International Research Fellow Awards: Hormonal Control of Seed Germination in Arabidopsis Thaliana

国际研究员奖：拟南芥种子萌发的激素控制

• 批准号: 9704233
• 负责人: Camille Steber
• 金额: $ 3.71万
• 依托单位: Fellowships
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9704233&HistoricalAwards=false
• 关键词: International; Research; Fellow; Awards; Hormonal

9704233 Steber The International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twenty four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Camille M. Steber to work with Dr. Peter D. McCourt at the University of Toronto in Toronto, Ontario. Dr. Steber will investigate the role of the plant hormone gibberellic acid (GA) in the control of seed dormancy and germination. To ensure that plant embryos survive the interval between release from the mother plant and germination, seeds accumulate nutrient reserves and become dormant and desiccation tolerant. There are mechanisms that ensure that seeds germinate only under "dormancy breaking" conditions that provide a beneficial environment for growth of the seedling. In studying the Arabidopsis thaliana, the plant hormone abscisic acid (ABA) is needed to establish dormancy while the GA is needed to end dormancy and induce seed germination. Little is known about the mechanisms by which GA induces germination. This project will focus on understanding those mechanisms governing GA receptivity in the seed using two genetic approaches: the first will identify GA-insensitive mutations using a screen for suppressors of an ABA-insensitive mutation; the second will identify genes which when overexpressed, suppress the inability of the GA auxotroph gal-3 to germinate in the absence of exogenous GA. Gaining this understanding of GA response will have important applications in the area of agriculture. Dr. McCourt is one of relatively few North American researchers who has investigated the role of both ABA and GA in the control of seed dormancy and germination. His laboratory at the University of Toronto will provide an opportunity for interaction with a wide- range of experts in plant biology including plant physiology, biochemistry, and development. ***

9704233 Steber 国际研究员奖励计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该项目的奖项提供了联合研究以及使用国外独特或互补的设施、专业知识和实验条件的机会。 该奖项将支持 Camille M. Steber 博士与 Peter D. McCourt 博士在安大略省多伦多市的多伦多大学进行为期 12 个月的博士后研究访问。 Steber 博士将研究植物激素赤霉酸 (GA) 在控制种子休眠和发芽中的作用。 为了确保植物胚胎在从母株释放到发芽之间的时间间隔内存活，种子积累营养储备并进入休眠和耐干燥状态。 有一些机制可确保种子仅在“打破休眠”的条件下发芽，从而为幼苗的生长提供有利的环境。 在研究拟南芥时，需要植物激素脱落酸 (ABA) 来建立休眠，而需要 GA 来结束休眠并诱导种子发芽。 关于 GA 诱导发芽的机制知之甚少。 该项目将侧重于使用两种遗传方法来了解控制种子中 GA 接受性的机制：第一个将使用筛选 ABA 不敏感突变的抑制子来识别 GA 不敏感突变；第二个将使用 ABA 不敏感突变的抑制子来识别 GA 不敏感突变。第二个将鉴定基因，当这些基因过度表达时，会抑制 GA 营养缺陷型 gal-3 在缺乏外源 GA 的情况下无法发芽。 了解遗传算法的响应将在农业领域产生重要的应用。 McCourt 博士是北美研究 ABA 和 GA 在控制种子休眠和发芽中作用的少数研究人员之一。 他在多伦多大学的实验室将提供与植物生物学（包括植物生理学、生物化学和发育）领域的广泛专家进行互动的机会。 ***