Interference of the viral effector proteins pp71 and IE1 with ND10-mediated intrinsic immunity against human cytomegalovirus infections (B03)

我们已经证明，一个特定的细胞器的细胞核，称为PML核体（PML-NB），被修改的病毒效应蛋白，如pp 71-或IE 1-蛋白的人巨细胞病毒。这与作为细胞限制因子的PML-NB组分的直接抑制功能的抑制作用相关。最近的研究揭示了PML-NB作为进一步先天性应答（例如IFN途径）的共调节结构的新兴作用。该提案研究了IE 1和pp 71如何能够逃避特别是受TRIM因子支配的内在和先天免疫应答。

We have demonstrated that a specific organelle of the cell nucleus, termed PML nuclear body (PML-NB), is modified by viral effector proteins such as the pp71- or IE1-protein of human cytomegalovirus. This correlates with antagonization of a direct repressive function of PML-NB components that act as cellular restriction factors. Recent studies reveal an emerging role of PML-NBs as co-regulatory structures of further innate responses (e.g. IFN pathway). This proposal investigates how IE1 and pp71 are able to evade intrinsic and innate immune responses particularly governed by TRIM factors.