CAA: Food Uptake in Marine Mixotrophic Protists

CAA：海洋混合营养原生生物的食物摄取

• 批准号: 9707423
• 负责人: Lucie Maranda
• 金额: $ 5.27万
• 依托单位: University of Rhode Island
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9707423&HistoricalAwards=false
• 关键词: CAA; Food; Uptake; Marine; Mixotrophic

This study will develop an innovative approach for the measurement of food uptake in marine mixotrophic dinoflagellates, with a long term research goal of contributing to the understanding of regulation and significance of mixotrophic processes in marine food webs. The objectives of this study are: 1. To use fluorescent membrane markers to label food vacuoles in live marine phagocytic and myzocytotic dinoflagellates as a measure of food uptake. 2. To relate the degree of fluorescence to the degree of food uptake. 3. To demonstrate the usefulness of such a method in detecting as well as measuring phagocytosis and myzocytosis in cultured dinoflagellates and in whole marine protist communities. This novel approach is independent of prey selection, prey density and the type of feeding protists.

本研究将为海洋兼养甲藻的食物摄取测量提供一种创新的方法，其长期研究目标是有助于理解海洋食物网中兼养过程的调控和意义。 本研究的目的是：1.利用荧光膜标记技术标记海洋甲藻的食物泡，作为食物摄取的一种测量方法。2.将荧光的程度与食物摄取的程度相关联。3.证明这种方法在检测和测量培养的甲藻和整个海洋原生生物群落的吞噬作用和myzocytosis的有用性。 这种新的方法是独立的猎物选择，猎物密度和类型的喂养原生生物。