CAA: Application of Molecular Data to Plant Evolutionary Biology

Deb 9707693 McDade这个项目将使PI获得执行分子生物学中的一些技术所需的技能，目标是将这些技术中的证据应用到她正在进行的植物进化生物学研究中。国际植物组织的项目尤其将受益于分子数据的获取：(1)大型开花植物科的系统发育关系和进化模式；(2)热带植物物种之间的杂交模式，以及这种杂交对进化和保护的影响；(3)北美西南部一系列山顶栖息地岛屿(美国亚利桑那州和新墨西哥州，以及墨西哥西北部的索诺拉州和奇瓦瓦州)上植物种群的进化分化模式。这项研究计划有两个关键组成部分。首先，PI将参观三个非常活跃的分子植物系统学实验室，这三个实验室在专业知识和兴趣方面相互补充。他们在史密森学会(分子系统学实验室，发起人E.Zimmer博士)和华盛顿大学(R.Olmstead博士和H.Bradshaw博士的实验室)。除了提供国际和平研究所寻求获得的关键技术方面的专业知识外，这些工作访问还将导致合作研究工作。其次，PI将在她所在机构的公共分子实验室实施她通过这些访问获得的所有技术。这一组成部分对于确保项目的长期成功是至关重要的，因为它增加了PI的研究工具套件。\\Clm-06\debpop\POPBIO\ABSTRACT\MCDADE.CAA.doc

DEB 9707693 McDade This project will enable the PI to acquire the skills needed to carry out a number of techniques in molecular biology with the goal of applying evidence from these techniques to her on-going research in plant evolutionary biology. In particular, the PI's projects that will benefit greatly from acquisition of molecular data are: (1) phylogenetic relationships and evolutionary patterns within the large flowering plant family Acanthaceae; (2) the pattern of hybridization between species of tropical plants, and the evolutionary and conservation implications of such hybridization; and (3) patterns of evolutionary differentiation among populations of plants that occur on a series of mountain top habitat islands in southwestern North America (U.S. states of Arizona and New Mexico, plus the northwestern Mexican states of Sonora and Chihuahua). The research plan has two key components. First, the PI will visit three very active laboratories of molecular plant systematics which complement each other in terms of expertise and interests. These are at the Smithsonian Institution (the Laboratory of Molecular Systematics, Dr. E. Zimmer, sponsor) and the University of Washington (laboratories of Drs. R. Olmstead and H. Bradshaw). In addition to providing expertise in key techniques that the PI seeks to acquire, these working visits will lead to collaborative research efforts. Second, the PI will implement all of the techniques that she acquires via these visits in a communal molecular laboratory at her home institution. This component is critical to ensure the long-term success of the project in adding to the PI's suite of research tools. \\Clm-06\debpop\POPBIO\ABSTRACT\MCDADE.CAA.doc