DNA Polymerase Recognition of Non-B DNA Templates

非 B DNA 模板的 DNA 聚合酶识别

• 批准号: 9714025
• 负责人: Susan Hardin
• 金额: $ 3万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9714025&HistoricalAwards=false
• 关键词: DNA; Polymerase; Recognition; Non; Templates

9714025 Hardin This Small Grant for Exploratory Research is based on the unexpected observation that a set of guanine-rich oligodeoxynucleotide primers as well as related telomeric sequences from yeast, Oxytricha and Tetrahymena are capable of priming DNA synthesis by Taq DNA polymerase in the absence of an independent DNA template. This research will determine the rules for base incorporation by DNA polymerase using non-B DNA template structures, which preliminary data indicates is very specific. Polymerase fidelity will be determined by cloning and sequencing products of multiple, independent polymerase chain reactions using a variety of primers, and the sequence data will be analyzed for consistency of base incorporation. Results from this research will facilitate development of a system to investigate the chemistry and enzymology of genome expansion as it relates to genome evolution, and may reveal an alternative mechanism for maintaining chromosome ends (telomers). This research will facilitate development of a system to investigate the chemistry and enzymology of genome expansion as it relates to genome evolution, and may reveal an alternative mechanism for maintaining chromosome ends (telomers).

小行星9714025哈丁 这项探索性研究的小额资助是基于一组富含鸟嘌呤的寡脱氧核苷酸引物以及来自酵母、尖毛藻和四膜虫的相关端粒序列的意外观察，这些引物能够在没有独立DNA模板的情况下通过Taq DNA聚合酶引发DNA合成。 本研究将确定DNA聚合酶使用非B DNA模板结构进行碱基掺入的规则，初步数据表明这是非常特异的。 将通过使用多种引物对多个独立聚合酶链反应的产物进行克隆和测序来确定聚合酶保真度，并将分析序列数据的碱基掺入一致性。 这项研究的结果将有助于开发一个系统来研究基因组扩增的化学和酶学，因为它与基因组进化有关，并可能揭示维持染色体末端（端粒）的另一种机制。 这项研究将促进一个系统的发展，以调查化学和酶学的基因组扩增，因为它涉及到基因组进化，并可能揭示一种替代机制，为维护染色体末端（端粒）。