Bacterial Regulation of Root Respiration

9722988菲利普斯土壤细菌已经进化出与植物根共存的复杂机制。重点研究了紫花苜蓿根瘤菌与紫花苜蓿的相互作用。这项工作是基于之前检测到的一种刺激根呼吸的细菌因子，尽管该因子在结构上与已知的参与结瘤的脂-甲壳素寡糖信号不同。此外，该因子不会引起树冠卷曲，其合成也不依赖于根的存在。这项工作将描述这一因素并确定其结构。不合成该因子的细菌突变株也将被描述为解开生物合成途径的特征。最后，纯化的化合物和细菌突变菌株将用于研究根定植的变化，共生伙伴之间的信号传递和植物反应。

9722988 Phillips Soil bacteria have evolved sophisticated mechanisms for coexisting with plant roots. The focus is on the interaction of Rhizobium meliloti and alfalfa. The work is based on the previous detection of a bacterial factor that stimulated root respiration, although the factor is structurally not identical to the known lipo-chitin oligosaccharide signals involved in nodulation. In addition, the factor does not induce roothair curling and its synthesis is not dependent on the presence of roots. The work will characterize this factor and determine its structure. Bacterial mutants which do not synthesize the factor will also be characterized to unravel the biosynthetic pathway. Finally, the purified compound and the bacterial mutant strains will be used to study alterations in root colonization, signaling between the symbiotic partners and plant responses.