Acetobacter diazotrophicus, an Endophyte of Sugarcane, Promotes Plant Growth: Studies of Nitrogen Fixation, Phytochormone Production, and the Colonization Process

固氮醋杆菌（一种甘蔗内生菌）促进植物生长：固氮、植物激素产生和定植过程的研究

• 批准号: 9728184
• 负责人: Christina Kennedy
• 金额: $ 31万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728184&HistoricalAwards=false
• 关键词: Acetobacter; diazotrophicus; Endophyte; Sugarcane; Promotes

9728184 Kennedy Acetobacter diazotrophicus is a nitrogen-fixing bacterium isolated from sugarcane grown throughout the world. It inhabits the interior spaces of sugarcane but not its outside surfaces or nearby soils. Evidence that this bacterium supplies fixed nitrogen for plant growth was obtained in the PI's laboratory: sugarcane plants inoculated with A. diazotrophicus grew better than did uninoculated plants or plants inoculated with mutants unable to fix nitrogen. This symbiosis provides a model system for studying the potential benefit of bacterial nitrogen fixation in grasses and cereals. The objectives are 1) to determine how much fixed N is supplied to the plants: 2) to learn whether production of growth hormones by A. diazotrophicus also benefits plant growth; and 3) to characterize how A. diazotrophicus colonizes sugarcane using bacteria tagged with fluorescent markers. The results will provide insight into how other monocots, such as corn, wheat or rice might benefit from nitrogen-fixing bacteria.

9728184 Kennedy Acetobacter diazotrophicus是一种从全世界种植的甘蔗中分离的固氮细菌。 它栖息在甘蔗的内部空间，而不是甘蔗的外表面或附近的土壤。 在PI的实验室中获得了这种细菌为植物生长提供固定氮的证据：固氮菌比未接种的植物或接种不能固氮的突变体的植物生长得更好。 这种共生关系为研究禾本科和谷类中细菌固氮的潜在效益提供了一个模型系统。 目的是：1）确定有多少固定氮供应给植物; 2）了解是否生产生长激素的A。diazotrophicus也有利于植物的生长; 3）研究A. diazotrophicus利用带有荧光标记的细菌定殖甘蔗。 研究结果将为其他单子叶植物，如玉米、小麦或水稻如何从固氮细菌中受益提供深入了解。