Quinate, Focal Point of Upstream Metabolism Leading to Lignin

奎宁酸,产生木质素的上游代谢的焦点

基本信息

  • 批准号:
    9728619
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing grant
  • 财政年份:
    1998
  • 资助国家:
    美国
  • 起止时间:
    1998-04-01 至 1999-09-10
  • 项目状态:
    已结题

项目摘要

9728619 Jensen and Bonner Quinic acid is one of the most abundant natural products in the biosphere. It is accumulated in some tissues of woody plants, e.g., newly forming needles of conifers, in amounts equaling up to 10% of the total dry weight. Quinic acid is exceedingly active as an intermediate which possesses alternative metabolic fates. In xylem-forming cells it is mainly transformed to phenylpropanoid precursors of lignin. In autotrophic cells, on the other hand, quinic acid is an effective precursor of other phenolic compounds - the most noteworthy ones being chlorogenic acid, gallic acid and protocatechuic acid. Chlorogenic acid and gallic acid confer resistance to herbivore predation and phytopathogenic microorganisms. Protocatechuic acid is catabolized to central intermediates and can serve as a carbon-source reserve during dark metabolism. Finally, quinic acid is a highly soluble, readily transported metabolite which can serve as a versatile precursor of aromatic amino acids in different tissue compartments. Loblolly pine (Pinus taeda), the experimental system, is under intensive study by others in order to understand and manipulate lignin content. Therefore, many biological and molecular-genetic resources are available to support this project, including a tissue-culture system which is lignin-inducible. The goal of this research is to elucidate the nature of the interfacing upstream metabolism which delivers L-phenylalanine to the phenylpropanoid metabolic section. It will focus upon three quinate dehydrogenase proteins: nQDH-NADP and nQDH-NAD in needle tissue and xQDH-NADP in xylem-forming cells. NQDH-NADP appears to be trifunctional, having catalytic domains which carry out the overall conversion of quinate to protocatechuate. The enzymes will be purified, used to raise specific antibodies, and characterized for physical and catalytic properties. cDNA clones corresponding to each QDH will be obtained by immunoscreening. Specific antibody will be used for immunogold EM cytoloca lization. Regulation will be examined by monitoring levels of enzyme activity, amounts of protein, and transcript abundance in response to developmental and environmental cues. The longterm objective is to understand how this entire metabolic network is differentially regulated to accomplish the dynamic alternative molecular fates of quinate. This is expected to provide ultimately a rational basis for biotechnological manipulations designed to modify lignin content by alteration of flux toward and away from quinate in different cellular compartments and in different specialized tissues.
9728619 Jensen 和 Bonner 奎尼酸是生物圈中最丰富的天然产物之一。它在木本植物的某些组织中积累,例如针叶树新形成的针叶,其含量高达总干重的 10%。奎尼酸作为具有替代代谢命运的中间体非常活跃。在木质部形成细胞中,它主要转化为木质素的苯丙素前体。另一方面,在自养细胞中,奎尼酸是其他酚类化合物的有效前体,其中最值得注意的是绿原酸、没食子酸和原儿茶酸。绿原酸和没食子酸赋予对草食动物捕食和植物病原微生物的抵抗力。原儿茶酸分解代谢为中心中间体,可作为暗代谢过程中的碳源储备。最后,奎尼酸是一种高度可溶、易于运输的代谢物,可作为不同组织区室中芳香族氨基酸的多功能前体。其他人正在对火炬松(Pinus taeda)这一实验系统进行深入研究,以了解和操纵木质素含量。因此,许多生物和分子遗传资源可用于支持该项目,包括木质素诱导的组织培养系统。本研究的目的是阐明将 L-苯丙氨酸输送到苯丙素代谢部分的接口上游代谢的性质。它将重点关注三种奎宁酸脱氢酶蛋白:针组织中的 nQDH-NADP 和 nQDH-NAD 以及木质部形成细胞中的 xQDH-NADP。 NQDH-NADP 似乎是三功能的,具有将奎宁酸完全转化为原儿茶酸的催化结构域。这些酶将被纯化,用于产生特异性抗体,并表征其物理和催化特性。通过免疫筛选获得对应于每个QDH的cDNA克隆。特异性抗体将用于免疫金电镜细胞定位。将通过监测响应发育和环境线索的酶活性水平、蛋白质含量和转录物丰度来检查调节。 长期目标是了解整个代谢网络如何进行差异调节以实现奎宁的动态替代分子命运。预计这最终将为生物技术操作提供合理的基础,该操作旨在通过改变不同细胞区室和不同专门组织中朝向和远离奎宁酸的通量来改变木质素含量。

项目成果

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Roy Jensen其他文献

Reliability Modeling in Electric Power Systems
Power System Economics
Characterization of Human Brain S100 Protein Fraction: Amino Acid Sequence of S100β
人脑 S100 蛋白组分的表征:S100β 的氨基酸序列
  • DOI:
  • 发表时间:
    1985
  • 期刊:
  • 影响因子:
    4.7
  • 作者:
    Roy Jensen;D. Marshak;Clint Anderson;T. Lukas;D. Watterson
  • 通讯作者:
    D. Watterson
The University of Kansas Cancer Center (KUCC), an NCI designated comprehensive cancer center
  • DOI:
    10.1186/s12943-023-01902-y
  • 发表时间:
    2023-11-28
  • 期刊:
  • 影响因子:
    33.900
  • 作者:
    Weijing Sun;Natalie Streeter;Joseph McGuirk;Roy Jensen
  • 通讯作者:
    Roy Jensen

Roy Jensen的其他文献

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{{ truncateString('Roy Jensen', 18)}}的其他基金

Quinate, Focal Point of Upstream Metabolism Leading to Lignin
奎宁酸,产生木质素的上游代谢的焦点
  • 批准号:
    9996414
  • 财政年份:
    1999
  • 资助金额:
    --
  • 项目类别:
    Continuing grant
NSF-NATO EAST EUROPE: Metabolic Interface Between Primary Secondary Metabolism in HigherPlants
NSF-NATO 东欧:高等植物初级次级代谢之间的代谢界面
  • 批准号:
    9355495
  • 财政年份:
    1993
  • 资助金额:
    --
  • 项目类别:
    Fellowship
Evolution of Aromatic Biosynthesis and Regulation in Prokaryotes
原核生物芳香生物合成和调控的进化
  • 批准号:
    8615314
  • 财政年份:
    1987
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
How Do Higher Plants Synthesize L-Phenylalanine
高等植物如何合成L-苯丙氨酸
  • 批准号:
    8309070
  • 财政年份:
    1984
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Evolution of Aromatic Biosynthesis and Regulation in Pro- Karyotes
原核生物芳香生物合成和调控的进化
  • 批准号:
    8316275
  • 财政年份:
    1984
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Enzymological Patterning: Indicator of Biochemical Phylogeny
酶学模式:生化系统发育的指标
  • 批准号:
    8208956
  • 财政年份:
    1982
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Biochemical Genetics of Aromatic Metabolism in Cultured Somatic Cells of Nicotiana Sylvestris
烟草培养体细胞芳香代谢的生化遗传学
  • 批准号:
    7907334
  • 财政年份:
    1979
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Gene-Enzyme Relationships in Somatic Cells
体细胞中的基因-酶关系
  • 批准号:
    7719929
  • 财政年份:
    1978
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Enzymological Diversity in Biochemical Pathways
生化途径的酶学多样性
  • 批准号:
    7812099
  • 财政年份:
    1978
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Pretyrosine Pathway of L-Tyrosine Biosynthesis
L-酪氨酸生物合成的前酪氨酸途径
  • 批准号:
    7619963
  • 财政年份:
    1976
  • 资助金额:
    --
  • 项目类别:
    Standard Grant

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