Electrochemical Biosensors Based on Quinoprotein Enzymes

基于醌蛋白酶的电化学生物传感器

• 批准号: 9728644
• 负责人: Hiroyasu Tachikawa
• 金额: $ 34.2万
• 依托单位: Jackson State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728644&HistoricalAwards=false
• 关键词: Electrochemical; Biosensors; Based; Quinoprotein; Enzymes

This research project, supported by the Analytical and Surface Chemistry Program, focusses on the development of quinoprotein enzyme based biosensors for the analysis of histamine, dopamine, and alcohols. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center and their students will study the preparation and characterization of biosensors using the enzymes methylamine dehydrogenase, aromatic amine dehydrogenase, and methanol dehydrogenase. Electrochemical methods, electrochemical quartz crystal microbalance measurements, and Raman spectroscopy will be used to characterize the structure and function of these enzyme based biosensors. This project is unique in using oxygen and NAD independent redox enzymes in biosensors and in the long-term medical applications are anticipated. This project will examine the utility of developing enzyme based biosensors that use oxygen and NA independent quinoprotein enzymes. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center will study the construction and characterization of these biosensors with their students. A broad, interdisciplinary approach to this project will involve use of electrochemical techniques, quartz crystal microbalance measurements, and Raman spectroscopy to determine the structure and properties of these new biosensors. Long-term goals focus on developing new applications for quantitative and qualitative analysis in medicine.

该研究项目在分析和表面化学计划的支持下，致力于开发基于藜麦蛋白酶的生物传感器，用于组胺、多巴胺和酒精的分析。杰克逊州立大学的Tchikawa教授和密西西比大学医学中心的Davidson教授及其学生将研究使用甲胺脱氢酶、芳香胺脱氢酶和甲醇脱氢酶的生物传感器的制备和表征。电化学方法、电化学石英晶体微天平和拉曼光谱将用于表征这些基于酶的生物传感器的结构和功能。该项目在生物传感器中使用氧气和NAD非依赖的氧化还原酶方面是独一无二的，在预期的长期医疗应用中也是独一无二的。该项目将研究开发基于酶的生物传感器的效用，该生物传感器使用氧气和NA非依赖的藜麦蛋白酶。杰克逊州立大学的立川教授和密西西比大学医学中心的戴维森教授将与他们的学生一起研究这些生物传感器的构造和特性。该项目的一个广泛的跨学科方法将涉及使用电化学技术、石英晶体微天平测量和拉曼光谱来确定这些新的生物传感器的结构和性能。长期目标侧重于开发医学定量和定性分析的新应用。