Electrochemical Biosensors Based on Quinoprotein Enzymes

该研究项目由分析和表面化学项目支持，重点开发基于藜蛋白酶的生物传感器，用于分析组胺，多巴胺和酒精。Jackson州立大学的Tachikawa教授和密西西比大学医学中心的Davidson教授及其学生将使用甲胺脱氢酶、芳香胺脱氢酶和甲醇脱氢酶来研究生物传感器的制备和特性。电化学方法、电化学石英晶体微天平测量和拉曼光谱将用于表征这些酶基生物传感器的结构和功能。该项目在生物传感器中使用氧气和NAD独立氧化还原酶方面是独一无二的，并有望在长期医学应用中得到应用。本项目将研究利用氧和NA不依赖的藜蛋白酶开发基于酶的生物传感器的效用。杰克逊州立大学的立川教授和密西西比大学医学中心的戴维森教授将和他们的学生一起研究这些生物传感器的结构和特性。该项目将采用广泛的跨学科方法，包括使用电化学技术、石英晶体微天平测量和拉曼光谱来确定这些新型生物传感器的结构和性能。长期目标侧重于开发定量和定性分析在医学中的新应用。

This research project, supported by the Analytical and Surface Chemistry Program, focusses on the development of quinoprotein enzyme based biosensors for the analysis of histamine, dopamine, and alcohols. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center and their students will study the preparation and characterization of biosensors using the enzymes methylamine dehydrogenase, aromatic amine dehydrogenase, and methanol dehydrogenase. Electrochemical methods, electrochemical quartz crystal microbalance measurements, and Raman spectroscopy will be used to characterize the structure and function of these enzyme based biosensors. This project is unique in using oxygen and NAD independent redox enzymes in biosensors and in the long-term medical applications are anticipated. This project will examine the utility of developing enzyme based biosensors that use oxygen and NA independent quinoprotein enzymes. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center will study the construction and characterization of these biosensors with their students. A broad, interdisciplinary approach to this project will involve use of electrochemical techniques, quartz crystal microbalance measurements, and Raman spectroscopy to determine the structure and properties of these new biosensors. Long-term goals focus on developing new applications for quantitative and qualitative analysis in medicine.