Improvement of a Biochemistry Laboratory Course

中央康涅狄格州立大学的科学课程旨在为学生提供高质量的指导和最先进的实验室经验，为工业和教育事业或进一步的研究生教育做准备。化学学士学位由美国化学会专业培训委员会（ACS CPT）认证。为了响应学生的兴趣，并更好地为学生做好工业准备，我们正在开发与ACS CPT指南一致的生物化学专业。为了实现这一目标，我们必须：(1)将我们目前的生物化学讲座从3学时延长到6学时；(2)通过结合现代技术和更多创新实验，提高3学时生物化学实验课程的范围和质量；(3)为对生物化学感兴趣的本科生提供更多的研究机会。本提案旨在通过购买改进的相控高压液相色谱（HPLC），高速，低温离心和组织均质化的仪器来改善生物化学实验室计划。这些仪器将在化学系的以下课程中使用：生物化学实验室和基础有机与生物化学；生物系的生态生理学。教授这些课程的教授也会在他们涉及本科生的个人研究项目中使用这些仪器。琼斯博士是化学系的生物化学家，正在研究多芳烃和卤代烃在原核生物和真核生物细胞中的代谢和毒性。她最近建立了一个小型组织培养实验室，用于在原代大鼠肝细胞培养中进行代谢研究。卡珀博士是生物系的比较生理学家，从事海洋和河口生物适应盐度的生理生化机制的研究。这些研究将通过高效液相色谱技术得到加强。具体来说，我们建议购买沃特斯公司具有紫外/可见和荧光检测能力的反相高效液相色谱系统。这将用于分析蛋白质水解物、氨基酸和其他生物分子，以及植物和动物组织中产生的环境代谢物。Sorval RC-26 Plus离心机速度26000 rpm)和铝固定角转子，将用于哺乳动物组织的亚细胞分离，包括用于代谢研究的钙沉淀微粒体，以及用于特定细胞蛋白质的梯度纯化。组织匀浆机将用于产生组织匀浆进行差动离心。这些仪器的获得将代表化学系生物化学培训的质量和范围的重大进步，并对该领域拟议的项目发展作出重大贡献。高效液相色谱系统将广泛应用于化学和生物科学系的其他本科生研究项目。

Science programs at Central Connecticut State University seek to provide students with high quality instruction and state of the art laboratory experiences in preparation for careers in industry and education or further postgraduate education. The B.S. degrees in Chemistry are certified by the American Chemical Society's Committee on Professional Training (ACS CPT). In response to student interest and to better prepare students for industry, we are developing a specialization in Biochemistry consistent with the ACS CPT guidelines. To achieve this goal we must (1) extend our current biochemistry lecture course from 3- to 6-credit hours; (2) improve the scope and quality of our 3-hour biochemistry laboratory program by the incorporation of modern technology and more innovative experiments; and (3) provide more research opportunities for undergraduates interested in biochemistry. The present proposal addresses our intent to improve the biochemistry laboratory program through the purchase of instrumentation for revised phased high pressure liquid chromatography (HPLC), high speed, low temperature centrifugation, and tissue homogenization. The instruments will be utilized in the following courses in the Chemistry Department: Biochemistry Laboratory and Basic Organic and Biological Chemistry; and in the Biology Department, in Ecological Physiology. The professors who instruct these courses will also utilize the instrumentation in their individual research programs which involve undergraduates. Dr. Jones, a biochemist in the Chemistry Department, is studying the metabolism and toxicity of polyaromatic hydrocarbons and halogenated hydrocarbons in prokaryote and eukaryote cells. She has recently established a small tissue culture laboratory for conducting metabolic studies in primary rat liver cell cultures. Dr. Kapper, a comparative physiologist in the Biology Department, is engaged in study of the physiological and biochemical mechanisms of salinity adaptation in marine and estuarine orga nisms. These studies would be enhanced by access to HPLC technology. Specifically, we propose to purchase a Waters reversed phase HPLC system with capability for uv/visible and fluorescence detection. This will be used for analyses of protein hydrolysates, amino acids and other biomolecules, and environmental metabolites generated in plant and animal tissues. The Sorval RC-26 Plus centrifuge (max. speed 26,000rpm) with and aluminum fixed-angle rotor, will be used for the subcellular fractionation of mammalian tissues including calcium-precipiated microsomes for metabolic studies, and for the gradient purification of specific cellular proteins. A tissue homogenizer will be used to generate tissue homogenates for differential centrifugation. The acquisition of these instruments would represent a major advancement in the quality and scope of training in biochemistry within the Chemistry Department, and contribute significantly to the proposed program development in this area. An HPLC system would be widely used in other as well as undergraduate research projects in the Departments of Chemistry and Biological Sciences.