POWRE: Metabolism of the Plant Hormone Indole-3-Acetic Acid During Somatic Embryogenesis

POWRE：体细胞胚胎发生过程中植物激素吲哚-3-乙酸的代谢

• 批准号: 9753001
• 负责人: Elaine Shea
• 金额: $ 7.4万
• 依托单位: Loyola University Maryland, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9753001&HistoricalAwards=false
• 关键词: POWRE; Metabolism; Plant; Hormone; Indole

Carrot cells in culture can be induced to form somatic embryos (no fertilization involved) by pre-treatment with auxin. When somatic embryogenesis occurs, cellular levels of indole-3-acetic acid (IAA) drop at least 10-fold and IAA synthesis switches to the non-tryptophan pathway. The amount of IAA in older embryos, its form (free or bound), its location in the embryo, and how these changes occur during development, are unknown. Understanding somatic embryogenesis is important for the propagation of rare plants and for genetic manipulation of plants for crop improvement. The aims of Dr. Shea's research project are to measure the levels of auxin in late stages of somatic embryo development, to examine the pathway of IAA biosynthesis in late stages of somatic embryo development, and to study the influence of exogenous auxin sources on embryogenesis. Dr. Shea is returning to active research after a long absence and is currently in a non-tenure-track teaching position while doing research voluntarily off-campus. Her goal is to obtain a tenure-track position with research opportunities. This POWRE grant will allow her to establish an active research program on campus that will include students, provide opportunities to attend scientific meetings and network with other scientists, and show potential employers that such plant research can attract extramural funding.

通过用生长素预处理，可以诱导培养中的胡萝卜细胞形成体细胞胚胎（不涉及受精）。 当体细胞胚胎发生时，吲哚-3-乙酸 (IAA) 的细胞水平下降至少 10 倍，并且 IAA 合成转换为非色氨酸途径。 较老胚胎中 IAA 的含量、其形式（游离或结合）、其在胚胎中的位置以及这些变化在发育过程中如何发生，目前尚不清楚。 了解体细胞胚胎发生对于稀有植物的繁殖和作物改良的植物遗传操作非常重要。 Shea博士的研究项目的目的是测量体细胞胚胎发育后期生长素的水平，检查体细胞胚胎发育后期IAA生物合成的途径，并研究外源生长素来源对胚胎发生的影响。 谢伊博士在长期缺席后重返活跃的研究领域，目前担任非终身教职，同时在校外自愿进行研究。 她的目标是获得具有研究机会的终身职位。 这笔 POWRE 赠款将使她能够在校园内建立一个积极的研究项目，其中包括学生，提供参加科学会议和与其他科学家建立联系的机会，并向潜在雇主表明此类植物研究可以吸引校外资金。