SBIR Phase I: Production and Application of a Beta-1,3- Polyglucon from a Gram Positive Bacterium

SBIR第一阶段：革兰氏阳性菌β-1,3-聚葡萄糖的生产和应用

• 批准号: 9761260
• 负责人: Eugene Butler
• 金额: $ 9.95万
• 依托单位: Genetic Architecture Education Analysis (GAEA), Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9761260&HistoricalAwards=false
• 关键词: SBIR; Phase; Production; Application; Beta

This Small Business Innovation Research Phase I project has the following objectives: (1) To genetically manipulate C. Flavigena to synthesize increased amounts of the KUSP1 polyglucan and (2) investigate the feasibility of new applications of the polymer. C. Flavigena is a gram positive bacterium; hence its useful beta-1,3-polyglucan product should not be contaminated with lipopolysaccharide endotoxins. The polymer is insoluble at neutral pH and soluble in mildly alkaline solutions; hence its gellation is easily controlled. Modern molecular biological techniques will be employed to develop a KUSP1 `over-producing` strain. In parallel we will examine the applicability of KUSP1-based gel systems for electrophoretic applications. The successful completion of this study will result in a ready supply of this polymer at an attractive cost for the commercial applications listed: a) Enhanced oil recovery via `in situ gellation` of the polymers in reservoirs, b) Encapsulation of medicines for `controlled release`, c) Encapsulation of agricultural chemicals, and d) Gelling agent/thickener/stabilizer in food products. This award is supported by the Small Business Innovation Research Program and the Experimental Program to Stimulate Competitive Research (EPSCoR).

该小型企业创新研究第一阶段项目有以下目标：(1) 通过基因操作 C. Flavigena 来合成更多量的 KUSP1 聚葡聚糖，以及 (2) 研究该聚合物新应用的可行性。 C. Flavigena是一种革兰氏阳性菌；因此，其有用的β-1,3-聚葡聚糖产品不应被脂多糖内毒素污染。 该聚合物在中性pH下不溶，溶于弱碱性溶液；因此其凝胶化很容易控制。 将采用现代分子生物学技术来开发KUSP1“过量生产”菌株。 与此同时，我们将研究基于 KUSP1 的凝胶系统在电泳应用中的适用性。 这项研究的成功完成将使该聚合物能够以极具吸引力的成本供应到下列商业应用：a) 通过聚合物在油藏中的“原位胶凝”提高石油采收率，b) 药物封装用于“控释”，c) 农用化学品封装，以及 d) 食品中的胶凝剂/增稠剂/稳定剂。 该奖项由小型企业创新研究计划和刺激竞争研究实验计划 (EPSCoR) 提供支持。