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The Role of Gap Junctions Expression in Adrenal Function

间隙连接表达在肾上腺功能中的作用

基本信息

批准号：
9808428
负责人：
Sandra Murray
金额：
$ 33.3万
依托单位：
University of Pittsburgh
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-09-01 至 2002-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808428&HistoricalAwards=false
关键词：
Role Gap Junctions Expression Adrenal

项目摘要

9808428 Murray Gap junctions between adrenal gland cells may provide a passageway for movement of regulatory molecules involved in adrenal function. Dr. Murray will test the hypothesis that gap junctions between adjacent adrenal cells participate in adrenal gland function and that gap junction expression is dependent on the functional state of the adrenal gland. Mice will be treated with compounds and/or procedures known to influence adrenal cell proliferation, steroidogenesis and morphology. Specifically, the effect of adrenocorticotropin (ACTH), dexamethasone, low sodium diet, hypophysectomy and adrenal regeneration on gap junctions will be studied in vivo (Objective 1). Adrenal glands from each of these treatment groups will be harvested for further study in cell culture (Objective 2). Transgeneic (lack Cx43 gap junction protein) mouse adrenal glands will e transplanted into adult mice. Adrenal gland viability, growth, steroidogenesis and cell proliferation will be measured in the adrenal transplants which either have or lack gap junctions (Objective 3). The protocols described are expected to increase our knowledge of the role of gap junctions in hormone responses. This information is critical to our understanding not only of hormone response in the adrenal gland but also to a general understanding of organ development, differentiation, and growth.

肾上腺细胞之间的Murray缝隙连接可能为参与肾上腺功能的调节分子的运动提供通道。 Murray博士将检验相邻肾上腺细胞之间的缝隙连接参与肾上腺功能以及缝隙连接表达依赖于肾上腺功能状态的假设。将用已知影响肾上腺细胞增殖、类固醇生成和形态的化合物和/或程序处理小鼠。具体而言，将在体内研究促肾上腺皮质激素（ACTH）、地塞米松、低钠饮食、垂体切除术和肾上腺再生对间隙连接的影响（目的1）。将收获这些给药组的肾上腺，用于进一步的细胞培养研究（目标2）。将转基因（缺乏Cx43间隙连接蛋白）小鼠肾上腺移植到成年小鼠中。在具有或缺乏间隙连接的肾上腺移植物中测量肾上腺活力、生长、类固醇生成和细胞增殖（目的3）。所描述的协议，预计将增加我们的知识的间隙连接在激素反应中的作用。这些信息不仅对我们理解肾上腺中的激素反应至关重要，而且对器官发育，分化和生长的一般理解也至关重要。