Symposium on Electron Crystallography of Macromolecules at Granlibakken Conference Center in Lake Tahoe, California

该奖项为1998年12月9日至12日在加州太浩湖的Granlibakken会议中心举行的高分子电子晶体学研讨会提供部分支持。本次研讨会的动机是基于结构生物学领域的最新进展，能够在3-10埃的分辨率范围内解析生物结构细节。研讨会将集中在电子晶体学的四个分支的最新进展，强调方法的发展和阐明大分子组装中的分子间相互作用。特别注意将支付给蛋白质-完整的膜组件和非膜蛋白质-在结晶单层;探测分子间的相互作用，通过拟合高分辨率的亚基结构到完整的复合物的低分辨率地图;在亚纳米分辨率的细丝和独立的颗粒分析;以及一种基于利用EM衍生的分子包络和非晶体学对称性的定相天然X射线数据的新方法。研讨会的目标是总结这些和相关的调查路线，评估进一步改进方法（更高的分辨率，加速分析）的前景，并促进其应用到更广泛的生物学问题。本次研讨会的另一个重要目的是向细胞和分子生物学界传播这一新兴的结构生物学工具。组织者将通过各种宣传方式吸引非结构生物学学科的与会者参加这次会议，并将会议安排在1998年在旧金山弗朗西斯科举行的细胞生物学年会之前。为了更广泛地传播，计划在《结构生物学杂志》上出版一个由受邀与会者提供的关于设想主题的特刊。

This award provides partial support for a Symposium on Electron Crystallography of Macromolecules at Granlibakken Conference Center in Lake Tahoe, California, between December 9-12, 1998. The motivation of this symposium is to be based on the recent advances in this field of structural biology capable of resolving biological structure details at 3-10 angstrom resolution range. The Symposium will focus on recent dramatic advances in four branches of electron crystallography, emphasizing both methodology development and the elucidation of inter-molecular interactions in large macromolecular assemblies. Particular attention will be paid to proteins - both integral membrane components and non-membrane proteins - in crystalline monolayers; probing inter-molecular interactions by fitting high resolution subunit structures into lower resolution maps of intact complexes; analysis of filaments and free-standing particles at subnanometer resolution; and a novel method of phasing native X-ray data based on exploiting EM-derived molecular envelopes and non-crystallographic symmetry. The goal of the Symposium is to summarize these and related lines of investigation, assess the prospects for further methodological improvement (higher resolution, accelerated analysis), and stimulate their application to a more extensive set of biological problems. Another important purpose of this Symposium is to disseminate this emerging structural biology tool to the cell and molecular biology community. The organizers will make an attempt to attract participants from non structural biology discipline attending this meeting via a variety of means of advertising the Symposium and scheduling it right before the 1998 Cell Biology Annual Conference to be held in San Francisco. For a broader dissemination, it is planned to publish a special issue of the envisaged topics in the Journal of Structural Biology contributed from the invited participants.