Structure and Function of Reproductive Fluid Proteins

9816747乳汁鱼能否成功受精并生产出可存活的后代取决于许多因素。重要的是将大量高度能动的、可存活的精子转移到雌性鱼类或存放在水中的卵子。本方案的目的是研究一种软骨鱼类体液中的结构和功能蛋白质，这些蛋白质可以增加精子的活力和松弛这些鱼类的子宫平滑肌。具体目标是：1)表征鱼类子宫平滑肌对雄性生殖液中新纯化的“raylasin”分子的松弛反应，并分离和表征其在子宫细胞细胞膜上的受体。2)鉴定和鉴定交配时鱼精子上分泌的液体中的精子刺激分子。将使用分析化学技术和显微技术来实现这些目标。这些研究的结果应在渔业管理中有实际应用，并有助于了解繁殖的基本生物学机制。

9816747LacySuccessful fertilization and production of viable offspring in fish is determined by a number of factors. Important is the transfer of large numbers of highly motile, viable sperm to the female fish or to the eggs deposited into the water. The objectives of the present proposal are to investigate the structure and function proteins in fluids of a cartilaginous fish species which increase sperm motility and relax these fish's uterine smooth muscle. Specific goals are to: 1) Characterize the relaxation response of the fish uterine smooth muscle to the newly purified "raylaxin" molecule in male reproductive fluids and to isolate and characterize its receptor in the cell membrane of the uterine cells. 2) Identify and characterize the sperm stimulatory molecules in the fluids secreted over the fish sperm at the time of mating. Analytical chemistry techniques and microscopy will be used to achieve these goals. The results of these studies should have practical application in fisheries' management as well as contribute to the knowledge of basic biological mechanisms of reproduction.