Gulf Coast Protein Crystallography Beamline (Development by Consortium)

墨西哥湾沿岸蛋白质晶体学光束线（由联盟开发）

• 批准号: 9871464
• 负责人: Robert Fox
• 金额: $ 175.06万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871464&HistoricalAwards=false
• 关键词: Gulf; Coast; Protein; Crystallography; Beamline

9871464Abstract This project involves the construction of a beam line for macromolecular crystallography at the Center for Advanced Microstructures and Devices (CAMD) in Baton Rouge, Louisiana. A consortium of crystallographers from Texas, Oklahoma and Louisiana and physicists at CAMD/LSU will design, construct and maintain a state-of-the-art protein crystallography beam line for multiwavelength anomalous dispersion (MAD) and other diffraction experiments at CAMD. Protein crystallography has provided invaluable insight into the molecular basis of life. These contributions range from a deep chemical understanding of enzyme mechanisms to the molecular basis of signal transduction and its role in cancer. An understanding of the molecular basis of biology will require structural information from many proteins. This is a daunting task because protein crystallography has traditionally been a challenging and labor-intensive methodology. Synchrotron radiation has aided macromolecular crystallography in several ways. The superior intensity over laboratory sources has allowed the determination of structures of large proteins and viruses that could not be previously contemplated. The development of the synchrotron-based multi-wavelength anomalous dispersion (MAD) technique has streamlined the structure determination process. The number of structures solved by this methodology has grown exponentially in the last few years, placing great and ever increasing demand on the very few synchrotron beamlines. Regular and fast access to beam lines through lengthy application and waiting procedures now hinders the solution of new structures and other structural studies in a serious way. The synchrotron at CAMD is in operation at an energy of 1.3-1.5 GeV. Together with an energy shifting wiggler which is being installed, the intensity of the beam for x-ray crystallography is estimated to be similar to that of a bending magnet at the National Synchrotron Light Source at Brookhaven National Laboratory. This should yield frame exposure times of under a minute for routine problems and up to 2 minutes for problem crystals. This is an order of magnitude faster than rotating anode times and has the extreme advantage of wavelength tunability for MAD experiments. The beamline will serve as a regional research and training facility. Its close proximity to the consortium laboratories will expedite the solution of new structures and allow students new to crystallography to participate alongside senior students and postdocs in solution of protein or nucleic acid structures. A general user program will be established to allow access to non-consortium crystallographers.

9871464摘要该项目涉及在路易斯安那州巴吞鲁日的先进微结构和器件中心（CAMD）建造一条用于大分子晶体学的光束线。 来自德克萨斯州、俄克拉荷马州和路易斯安那州的晶体学家以及CAMD/LSU的物理学家组成的联盟将设计、建造和维护一条最先进的蛋白质晶体学光束线，用于CAMD的多波长异常色散（MAD）和其他衍射实验。 蛋白质晶体学为了解生命的分子基础提供了宝贵的见解。 这些贡献的范围从酶机制的深入化学理解到信号转导的分子基础及其在癌症中的作用。 要了解生物学的分子基础，需要许多蛋白质的结构信息。 这是一项艰巨的任务，因为蛋白质晶体学传统上是一种具有挑战性且劳动密集型的方法。 同步辐射在几个方面帮助了大分子晶体学。 相对于实验室来源的上级强度允许确定以前不能考虑的大蛋白质和病毒的结构。 基于同步加速器的多波长反常色散（MAD）技术的发展简化了结构测定过程。 在过去的几年里，通过这种方法解决的结构数量呈指数级增长，对非常少的同步加速器光束线提出了巨大且不断增长的需求。 通过冗长的申请和等待程序定期和快速地访问梁线，现在严重阻碍了新结构和其他结构研究的解决方案。CAMD的同步加速器在1.3-1.5 GeV的能量下运行。 加上正在安装的能量转移摆动器，用于X射线晶体学的光束强度估计与布鲁克海文国家实验室国家同步加速器光源的偏转磁铁强度相似。 这将产生低于一分钟的常规问题和长达2分钟的问题晶体帧曝光时间。 这比旋转阳极时间快一个数量级，并且对于MAD实验具有波长可调谐性的极端优势。光束线将作为区域研究和培训设施。 它靠近联盟实验室，将加快新结构的解决方案，并允许晶体学新学生与高年级学生和博士后一起参与蛋白质或核酸结构的解决方案。 将建立一个通用用户程序，以允许非联盟晶体学家访问。