Gulf Coast Protein Crystallography Beamline (Development by Consortium)

本项目涉及在路易斯安那州巴吞鲁日先进微结构与器件中心（CAMD）建设用于大分子晶体学的束流线。来自德克萨斯州、俄克拉何马州和路易斯安那州的晶体学家和CAMD/LSU的物理学家组成的联盟将为CAMD的多波长异常色散（MAD）和其他衍射实验设计、构建和维护一条最先进的蛋白质晶体学光束线。蛋白质晶体学为了解生命的分子基础提供了宝贵的见解。这些贡献范围从对酶机制的深刻化学理解到信号转导的分子基础及其在癌症中的作用。要了解生物学的分子基础，就需要了解许多蛋白质的结构信息。这是一项艰巨的任务，因为蛋白质晶体学传统上是一种具有挑战性和劳动密集型的方法。同步辐射在几个方面对大分子晶体学有帮助。与实验室源相比，优越的强度使得以前无法考虑的大型蛋白质和病毒结构的测定成为可能。基于同步加速器的多波长异常色散（MAD）技术的发展简化了结构测定过程。在过去的几年中，通过这种方法解决的结构数量呈指数级增长，对极少数同步加速器光束线提出了巨大且不断增长的需求。通过冗长的申请和等待程序，定期和快速地访问梁线，现在严重阻碍了新结构和其他结构研究的解决方案。CAMD的同步加速器以1.3-1.5 GeV的能量运行。加上正在安装的能量转移摆动器，用于x射线晶体学的光束强度估计与布鲁克海文国家实验室国家同步加速器光源的弯曲磁铁的强度相似。这应该产生的框架曝光时间不到一分钟的常规问题和高达2分钟的问题晶体。这比旋转阳极时间快了一个数量级，并且在MAD实验中具有波长可调性的极大优势。该光束线将作为区域研究和培训设施。它靠近联盟实验室，将加快新结构的解决方案，并允许新的结晶学学生与高年级学生和博士后一起参与蛋白质或核酸结构的解决方案。将建立一个一般用户程序，以允许非联盟晶体学家访问。

9871464Abstract This project involves the construction of a beam line for macromolecular crystallography at the Center for Advanced Microstructures and Devices (CAMD) in Baton Rouge, Louisiana. A consortium of crystallographers from Texas, Oklahoma and Louisiana and physicists at CAMD/LSU will design, construct and maintain a state-of-the-art protein crystallography beam line for multiwavelength anomalous dispersion (MAD) and other diffraction experiments at CAMD. Protein crystallography has provided invaluable insight into the molecular basis of life. These contributions range from a deep chemical understanding of enzyme mechanisms to the molecular basis of signal transduction and its role in cancer. An understanding of the molecular basis of biology will require structural information from many proteins. This is a daunting task because protein crystallography has traditionally been a challenging and labor-intensive methodology. Synchrotron radiation has aided macromolecular crystallography in several ways. The superior intensity over laboratory sources has allowed the determination of structures of large proteins and viruses that could not be previously contemplated. The development of the synchrotron-based multi-wavelength anomalous dispersion (MAD) technique has streamlined the structure determination process. The number of structures solved by this methodology has grown exponentially in the last few years, placing great and ever increasing demand on the very few synchrotron beamlines. Regular and fast access to beam lines through lengthy application and waiting procedures now hinders the solution of new structures and other structural studies in a serious way. The synchrotron at CAMD is in operation at an energy of 1.3-1.5 GeV. Together with an energy shifting wiggler which is being installed, the intensity of the beam for x-ray crystallography is estimated to be similar to that of a bending magnet at the National Synchrotron Light Source at Brookhaven National Laboratory. This should yield frame exposure times of under a minute for routine problems and up to 2 minutes for problem crystals. This is an order of magnitude faster than rotating anode times and has the extreme advantage of wavelength tunability for MAD experiments. The beamline will serve as a regional research and training facility. Its close proximity to the consortium laboratories will expedite the solution of new structures and allow students new to crystallography to participate alongside senior students and postdocs in solution of protein or nucleic acid structures. A general user program will be established to allow access to non-consortium crystallographers.