CAREER: Synthesis, Characterization and Application of Fluorescent Nanolipobeads for Optochemical Sensing in Single Living Cells

新奥尔良大学的Z. Rosenzweig教授在分析和表面化学项目的支持下，研究了一种新的基于荧光的分析方法，用于测定单个活细胞中的pH值、分子氧和过氧化氢的水平。与荧光显微镜或细胞细胞术等方法相比，新方法的侵入性更小，速度更快，并且建议用户较少涉及技术。这些测量表明细胞暴露于氧化剂中，通过在细胞内形成活性氧对细胞有害。测量是通过监测纳入细胞膜的纳米脂珠的荧光发射来完成的。CAREER计划的教育方面通过对大一化学实验室的实验室部分的拟议修订和将临床化学实验纳入分析化学研究生课程来解决。个体细胞被认为是环境污染物诱导的生物应激的准确和敏感的指标。罗森茨威格教授的项目开发了非常小的荧光珠，这些荧光珠被整合到细胞膜中，并提供一种反应，作为细胞内由于压力而产生的活性氧水平的指示器。

This project of Professor Z. Rosenzweig of the University of New Orleans, supported by the Analytical and Surface Chemistry program, is a study of a new fluorescence based analytical method for determination of pH, and the levels of molecular oxygen and hydrogen peroxide in single living cells. The new method is less invasive and more rapid than methods such as fluorescence microscopy or cell cytometry, and is proposed to be less technically involved for the user. The measurements are indicative of exposure of the cell to oxidizing agents that are shown to be detrimental to the cell via formation of reactive oxygen species within the cell. The measurement is made by monitoring the fluorescent emission of nanolipobeads that are incorporated in the cell membranes. The educational aspects of the CAREER program are addressed via proposed revisions to the laboratory portion of the freshman chemistry laboratory and the incorporation of clinical chemistry experiments into an analytical chemistry graduate course.Individual cells are considered to be accurate and sensitive indicators of biological stress induced by environmental pollutants. The project of Professor Rosenzweig develops very small fluorescent beads that are incorporated into the cell membrane and provide a response that is an indicator of levels of reactive oxygen species inside the cell that are produced as a result of stress.