CAREER: The Design of Synthetic Gene Transfer Agents

本提案的目标是开发基于脂质体和基于合成聚合物载体的足够的结构和动态数据，以允许设计更有效的基因转移剂。将从数据中导出一个理论模型，其中包括：两种载流子类型的相图，确定平衡结构作为系统参数的函数；检查可能的非平衡结构及其形成条件；以及使用结构/功能关系将各种载体的性能联系起来。本提案的最终目标是确定和量化通过合成载体控制基因转移的基本过程。

The goal of this proposal is to develop sufficient structural and dynamic data on liposome-based and synthetic polymer-based vectors to permit the design of more efficient gene transfer agents. A theoretical model will be derived from the data which includes:Phase diagrams of both carrier types identifying the equilibrium structures as a function of system parameters;Examining possible non-equilibrium structures and the conditions for their formation; and Relating the performance of the various carriers using structure/function relationships.The ultimate goal of this proposal is to identify and quantify the fundamental processes controlling gene transfer via synthetic carriers.